GFP Mouse anti-Tag, Biotin, Clone: 9F9.F9, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Mouse Serum
• Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA
• No reaction is seen against RFP
• The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal
• GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms
• As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted
• GFP technology has revealed considerable new insights in the physiological activities of living cells
• GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment
• The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors
• GFP fluorescence is stable, species independent and is suitable for a variety of applications
• GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization
• Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.