Telomerase catalytic subunit Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum
• Although it has been reported that this antibody reacts with mouse TERT (mTERT) (see Drissi, et al
• 2001), the binding to mTERT is considerably weaker and less specific than the binding to hTERT (not shown)
• Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG
• The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat
• Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres
• Deregulation of telomerase expression in somatic cells may be involved in oncogenesis
• Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks
• Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase (TERT) have been identified; the full-length sequence of some variants has not been determined
• Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.