NEK2Rabbit anti-Human, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• BLAST analysis indicates 100 % homology of the immunizing sequence with NEK2 homologues from human and chimpanzee
• Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence
• NEK2 is closely related in its catalytic domain to the serine/threonineprotein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2
• Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2
• These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression
• Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation
• Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides
• NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers
• This antibody is specific to isoform 2 of NEK2.