ATF3Rabbit anti-Human, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity-purified antibody is directed against human ATF3 and is useful in determining its presence in various assays including ELISA and western blotting
• This polyclonal antibody recognizes over-expressed ATF3 protein found in various expression systems
• Reactivity is observed against human ATF3
• Cross reactivity with ATF3 from other mammalian sources has not been tested
• ATF3 (Activating transcription factor 3) is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors
• It encodes a protein with a calculated molecular mass of 22 kD
• ATF3 represses rather than activates transcription from promoters with ATF binding elements
• An alternatively spliced form of ATF3 (ATF3 delta Zip) encodes a truncated form ATF3 protein lacking the leucine zipper protein-dimerization motif and does not bind to DNA
• In contrast to ATF3, ATF3 delta Zip stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter
• It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes
• Three transcript variants encoding two different isoforms have been found for this gene.