Phospho-APC1 (Ser377)Rabbit anti-Human, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This antibody is specific for phosphorylated human APC1 protein at the pS377 residue
• A BLAST analysis was used to suggest reactivity with this protein from human, dog, rat, and bovine based on 100% homology for the immunogen sequence
• Cross reactivity with APC1 protein from mouse is expected, as this sequence show 90% homologous between human and mouse
• Cross reactivity with APC1 homologues from other sources has not been determined
• Minimal reactivity is expected with the non-phosphorylated form of the protein
• Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis
• APC1, also known as mitotic checkpoint regulator (MCPR), is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle
• APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence
• The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex.