FIV matrix protein p15Rabbit anti-Virus, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Directed against FIV Matrix Protein p15
• The Gag family is characterized by assembly of the viral capsid at either the plasma membrane or at the limiting membrane of late endosomes
• The capsid assembles from the viral Gag polyprotein
• Upon release of a budding virion, Gag precursor protein is cleaved by the viral protease into its mature products, namely Matrix Protein, Capsid and Nucleocapsid
• Matrix Protein, located at the N-terminus of the Gag polyprotein, is usually myristylated during protein translation, prior to the later events of virus assembly
• The myristate moiety is believed to be sequestered within the Matrix Protein during protein translation and later facilitates membrane binding upon exposure resulting from conformational changes
• Essential functions attributed to the Matrix Protein of lentiviruses include targeting newly synthesized Gag precursor proteins to the site of virus assembly by binding with cellular components such as phosphatidylinositides
• In the mature virus particle, the Matrix Protein provides internal structure to the virion within the capsid, but is not exposed at the surface of the particle
• Based on studies with HIV, it is postulated that FIV Matrix Protein may also serve additional functions, including nuclear localization of the viral core upon entry of the virus into a new host cell.