50-199-3730
His-Tag Recombinant Mouse Monoclonal Antibody, AbFlex™, Active Motif™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-199-3730-Each-of-1 | In Stock | ₹ 8,727.12 |
50-199-3730 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 8,727.12
GST (18%): ₹ 1,570.882
Total Price: ₹ 10,298.002
Antigen
His-Tag
Classification
Monoclonal
Conjugate
Unconjugated
Gene Alias
6His tag; 6X His; His Tag; Histidine Tag
Immunogen
The His-Tag is comprised of a series of six or more histidine residues that are used to label the carboxy or amino terminus of a recombinant protein. This antibody is designed to recognize the His-Tag (HHHHHH).
Quantity
10 μg
Primary or Secondary
Primary
Product Type
Antibody
Isotype
IgG2a
Applications
Western Blot
Concentration
1 mg/mL
Formulation
140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Host Species
Mouse
Purification Method
Protein A
Regulatory Status
RUO
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Form
Liquid
Target Molecule
Tag
Related Products
Description
- The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH)
- The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography
- Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering
- By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available
- His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins
- For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
- His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
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Antigen: His-Tag
Classification: Monoclonal
Conjugate: Unconjugated
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Immunogen: The His-Tag is comprised of a series of six or more histidine residues that are used to label the carboxy or amino terminus of a recombinant protein. This antibody is designed to recognize the His-Tag (HHHHHH).
Quantity: 10 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a
Applications: Western Blot
Concentration: 1 mg/mL
Formulation: 140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Host Species: Mouse
Purification Method: Protein A
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: Tag
Antigen:
His-Tag
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Immunogen:
The His-Tag is comprised of a series of six or more histidine residues that are used to label the carboxy or amino terminus of a recombinant protein. This antibody is designed to recognize the His-Tag (HHHHHH).
Quantity:
10 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a
Applications:
Western Blot
Concentration:
1 mg/mL
Formulation:
140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Host Species:
Mouse
Purification Method:
Protein A
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
Tag
Antigen: 5-methylcytosine
Classification: Monoclonal
Conjugate: Unconjugated
Gene Alias: 5mc; 5-mC; 5-meC; 5-Methyl-cytosine; C5H7NO3
Immunogen: This antibody was raised against 5-methylcytosine conjugated to kLH.
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a
Applications: Dot Blot
Concentration: 1 mg/mL
Formulation: 140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Host Species: Mouse
Purification Method: Protein A
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: Chemical
Antigen:
5-methylcytosine
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Alias:
5mc; 5-mC; 5-meC; 5-Methyl-cytosine; C5H7NO3
Immunogen:
This antibody was raised against 5-methylcytosine conjugated to kLH.
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a
Applications:
Dot Blot
Concentration:
1 mg/mL
Formulation:
140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Host Species:
Mouse
Purification Method:
Protein A
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
Chemical
Antigen: 5-methylcytosine
Classification: Monoclonal
Conjugate: Unconjugated
Gene Alias: 5mc; 5-mC; 5-meC; 5-Methyl-cytosine; C5H7NO3
Immunogen: This antibody was raised against 5-methylcytosine conjugated to kLH.
Quantity: 10 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a
Applications: Dot Blot
Concentration: 1 mg/mL
Formulation: 140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Host Species: Mouse
Purification Method: Protein A
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: Chemical
Antigen:
5-methylcytosine
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Alias:
5mc; 5-mC; 5-meC; 5-Methyl-cytosine; C5H7NO3
Immunogen:
This antibody was raised against 5-methylcytosine conjugated to kLH.
Quantity:
10 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a
Applications:
Dot Blot
Concentration:
1 mg/mL
Formulation:
140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Host Species:
Mouse
Purification Method:
Protein A
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
Chemical
Antigen: Histone H3K27ac
Classification: Monoclonal
Conjugate: Unconjugated
Gene Alias: Acetyl H3; Acetyl Histone H3; Acetyl-H3 K27; Acetyl-H3 Lys27; Acetyl-Histone H3 K27; Acetyl-Histone H3 Lys27; H3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3 histone, family 3C; H3 K27ac; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3.3A; H3.3b; H3.5; H3/A; H3/b; H3/d; H3/f; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3-143; H3-291; H3-3A; H3-3B; H3-53; H3-614; H3a; H3b; H3-B; H3c1; H3c10; H3c11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3c8; H3f; H3-F; H3F1K; H3F2; H3F3; H3F3A; H3f3b; H3F3C; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; H3k27; H3Lys27ac; Hist1; Hist1h3a; HIST1H3B; Hist1h3c; HIST1H3D; Hist1h3e; HIST1H3F; HIST1H3G; HIST1H3H; Hist1h3i; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1;
Immunogen: This antibody was raised against a synthetic peptide including acetyl-lysine 27 of human histone H3.
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a
Applications: ChIP sequencing (ChIP-seq), ELISA, Western Blot
Concentration: 1 mg/mL
Formulation: 140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Host Species: Mouse
Purification Method: Protein A
Regulatory Status: RUO
Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
Form: Liquid
Target Molecule: __
Antigen:
Histone H3K27ac
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Alias:
Acetyl H3; Acetyl Histone H3; Acetyl-H3 K27; Acetyl-H3 Lys27; Acetyl-Histone H3 K27; Acetyl-Histone H3 Lys27; H3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3 histone, family 3C; H3 K27ac; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3.3A; H3.3b; H3.5; H3/A; H3/b; H3/d; H3/f; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3-143; H3-291; H3-3A; H3-3B; H3-53; H3-614; H3a; H3b; H3-B; H3c1; H3c10; H3c11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3c8; H3f; H3-F; H3F1K; H3F2; H3F3; H3F3A; H3f3b; H3F3C; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; H3k27; H3Lys27ac; Hist1; Hist1h3a; HIST1H3B; Hist1h3c; HIST1H3D; Hist1h3e; HIST1H3F; HIST1H3G; HIST1H3H; Hist1h3i; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1;
Immunogen:
This antibody was raised against a synthetic peptide including acetyl-lysine 27 of human histone H3.
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a
Applications:
ChIP sequencing (ChIP-seq), ELISA, Western Blot
Concentration:
1 mg/mL
Formulation:
140mM HEPES, 35mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
Host Species:
Mouse
Purification Method:
Protein A
Regulatory Status:
RUO
Content And Storage:
-20°C, Avoid Freeze/Thaw Cycles
Form:
Liquid
Target Molecule:
__