CD61 (Integrin beta 3) Monoclonal Antibody (VI-PL2), APC, eBioscience™, Invitrogen™

Description

• Description: The VI-PL2 monoclonal antibody reacts with human CD61, also known as integrin beta 3 and GPIIIa
• CD61 is a 90-110 kDa member of the beta integrin family expressed by a wide variety of cells, including leukocytes, platelets, endothelial and smooth muscle cells
• CD61 binds non-covalently with the alpha integrins CD41 and CD51, to form the alpha IIb beta3 (CD41/CD61) and alpha v beta3 (CD51/CD61) complexes
• These alpha beta heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation
• The CD41/CD61 integrin has been shown to be involved in platelet aggregation, and binds to fibrinogen, von Willebrand Factor (vWF) and fibronectin
• The CD51/CD61 integrin binds to matrix proteins including vitronectin, fibronectin, vWF and fibrinogen, and has been shown to have a strong role in modulating the migration and survival of angiogenic endothelial cells
• This VI-PL2 antibody has also been shown to crossreact with canine and several non-human primate species, including baboon, rhesus, and cynomologus monkey
• Applications Reported: This VI-PL2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This VI-PL2 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets
• This can be used at 5 μL (0.25 μg) per test
• CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors
• CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin
• CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface
• CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51
• CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin
• The CD61 protein product is composed of an alpha chain and a beta chain
• A given chain may combine with multiple partners resulting in different integrins
• CD61 is found along with the alpha IIb chain in platelets
• Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling
• Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.