CD45RA Monoclonal Antibody (GRT22), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: This GRT22 monoclonal antibody reacts with human and non-human primate CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ and CD8+ naive peripheral T lymphocytes and B cells
• The GRT22 antibody crossreacts to rhesus CD45RA
• Crossblocking studies indicate that GRT22 and HI100 recognize different epitopes
• Applications Reported: This GRT22 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This GRT22 antibody has been pre-titrated and tested by flow cytometric analysis of normal human and rhesus peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
• CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
• CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
• CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains
• CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
• Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
• CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues
• It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions
• Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.