CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein
• CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth
• CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes
• Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71
• Expression level differences have been observed in different mouse strains
• Applications Reported: This R17217 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This R17217 (RI7 217.1.4) antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes
• Endosomal acidification leads to iron release
• The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor
• Transferrin receptor is necessary for development of erythrocytes and the nervous system
• Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.