IL-8 (1-77) (CXCL8) Monoclonal Antibody (8CH), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: This 8CH monoclonal antibody reacts with human IL-8 (CXCL8), a pro-inflammatory CXC chemokine
• It is synthesized as a 99 amino acid precursor protein that is further processed into one of four isoforms, with the most common being 72 or 77 amino acids in length
• IL-8(77) is secreted primarily by endothelial cells and is thought to be a less potent neutrophil activator than the other forms
• It is present at high levels during fetal development, where it mediates angiogenesis rather than inflammation
• The predomit form present in adults is IL-8(72), which is expressed by monocytes, macrophages, epithelial cells, and fibroblasts in response to inflammatory stimuli, environmental stress, and steroid hormones
• IL-8(72) is essential for the activation and recruitment of neutrophils to sites of inflammation, and has also been found to influence T cell migration
• Signaling occurs through the G-protein coupled receptors CXCR1 or CXCR2
• IL-8 transcripts are often upregulated in tumors, and it is associated with tumor angiogenesis and metastasis
• Applications Reported: This 8CH antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This 8CH antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis
• This can be used at 5 μL (0.03 μg) per test
• Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB
• IL-8 functions as a chemoattractant and potent angiogenic factor
• The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells
• In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies
• IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors
• Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C
• IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q
• Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.