CD8b Monoclonal Antibody (SIDI8BEE), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The SIDI8BEE monoclonal antibody reacts with human and rhesus macaque CD8 beta
• The CD8b chain associates with the CD8a chain to form the CD8ab heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells
• The CD8 receptor complex binds to MHC class I and plays a role in T cell development and activation of mature T cells
• Long been thought to exist either as a heterodimer of the alpha and beta chain or as a homodimer of alpha chains, beta chains have now been shown to homodimerize as well
• SIDI8BEE can detect the CD8 beta chain in both the CD8ab heterodimer and the CD8bb homodimer
• SIDI8BEE is cross-reactive to human and rhesus macaque CD8b
• Other non-human primate species have not been tested
• Applications Reported: This SIDI8BEE antibody has been reported for use in flow cytometric analysis
• Applications Tested: This SIDI8BEE antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood lymphocytes
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system
• The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules
• The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain
• Both alpha and beta chains share significant homology to immunoglobulin variable light chains
• This gene encodes the CD8 beta chain isoforms
• Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described
• A pseudogene, also located on chromosome 2, has been identified.