CD221 (IGF1R) Monoclonal Antibody (1H7), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: This 1H7 monoclonal antibody reacts with human insulin-like growth factor-1 receptor (IGF-1R), also known as CD221
• This receptor is a cell surface-expressed glycoprotein composed of two extracellular alpha subunits and two transmembrane beta subunits that possess tyrosine kinase activity
• Expressed on nearly all cell types, this receptor binds IGF-I and IGF-II, as well as insulin
• Ligand binding leads to activation of the PI3K/Akt and MAPK pathways, which mediate cell proliferation and survival
• Many tumors and transformed cells display altered IGF-1R expression
• This monoclonal antibody has been reported to block binding of IGF-I and IGF-II to the IGF-1R
• Applications Reported: This 1H7 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 1H7 antibody has been pre-titrated and tested by flow cytometric analysis of the HeLa cell line
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2
• IGFR has been implicated in a variety of cancers
• The protein is a tetramer consisting of two alpha, and two beta subunits
• The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2
• Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136
• The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.