CD147 Monoclonal Antibody (8D12), PE, eBioscience™, Invitrogen™

Description

• The monoclonal antibody 8D12 recognizes CD147 which is a member of the immunoglobulin superfamily
• It is also called EMMPRIN and basigin
• CD147 has been characterized as a tumor cell collagenase stimulating molecule and has also been shown to have a role in cell adhesion
• CD147 can be found in association with integrins alpha3 beta1 and alpha6 beta1 by co-immpunopreciptations
• Additionally it is thought to play a role in T cell activation and thymocyte cycling
• CD147 is expressed broadly on hematopoietic and non-hematopoietic cells, especially in tumor cells
• Applications Reported: This 8D12 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 8D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6, EMMPRIN, extracellular matrix metalloproteinase inducer, TCSF, tumour cell-derived collagenase-stimulatory factor) is a 50-60 kDa glycoprotein with multiple glycosylated forms
• CD147 is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression
• CD147 is also a member of the immunoglobulin superfamily
• The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumor cells
• CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.