PDGF-BB Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PDGF-BB
• Anti-Human PDGF-BB-specific antibody was purified by affinity chromatography employing an immobilized Human PDGF-BB matrix
• Sandwich ELISA: To detect Human PDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PDGF-BB (500-P47Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PDGF-BB
• Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• The protein encoded by this gene is a member of the platelet-derived growth factor family
• The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines
• This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds
• Mutations in this gene are associated with meningioma
• Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor
• Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.