Dilution:
Western Blot 1 - 2 ug/ml, Simple Western, Flow Cytometry, Immunohistochemistry 1:250, Immunocytochemistry/Immunofluorescence 1:50 - 1:200, Immunoprecipitation reported by customer review, Immunohistochemistry-Paraffin 1:250-1:500, Immunohistochemistry-Frozen 1:250, In vivo assay reported in scientific literature (PMID 32173468), Immunofluorescence 1:50 - 1:200, CyTOF-ready, Knockout Validated
Classification:
Monoclonal
Target Species:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Primate, Xenopus species
Gene Alias:
All-trans-retinyl-palmitate hydrolase, EC 3.1.1.64, EC 5.2.1.7, LCA2p63, mRPE65, RBP-binding membrane protein, rd12, Retinal pigment epithelium-specific 65 kDa protein, retinal pigment epithelium-specific protein (65kD), retinal pigment epithelium-specific protein 65kDa, retinitis pigmentosa 20 (autosomal recessive), retinoid isomerohydrolase, Retinol isomerase, RP20, sRPE65
Purification Method:
Protein G purified
Test Specificity:
NB100-355 is specific for RPE65.
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), In vitro Assay, CyTOF
Research Discipline:
Cellular Markers, Lipid and Metabolism, Neuronal Cell Markers, Neuroscience, Sensory Systems, Signal Transduction, Vision
Immunogen:
This RPE65 Antibody (401.8B11.3D9) was developed against bovine RPE65 microsomal membrane proteins. [UniProt# Q28175]
Primary or Secondary:
Primary
Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.