IGF1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-I
• Anti-Human IGF-I-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect Human IGF-I by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-I (500-P11) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-I
• Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P11BT-1MG will be provided as 2 x 500 μg IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity
• A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival
• Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue
• IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake
• In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues
• IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death
• Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins
• Low levels of IGF1 have been linked to Alzheimer's disease
• IGF1 is processed from a precursor, bound by a specific receptor, and secreted
• Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.