FGF17 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-17
• Anti-Human FGF-17-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-17 matrix
• Sandwich ELISA: To detect Human FGF-17 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-17 (500-P152Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-17
• Western Blot: To detect Human FGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-17 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family
• FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion
• This gene was shown to be prominently expressed in the cerebellum and cortex
• The mouse homolog of this gene was localized to specific sites in the midline structures of the forebrain, the midbrain-hindbrain junction, developing skeleton and developing arteries, which suggests a role in central nervous system, bone and vascular development
• This gene was referred to as FGF-13 in reference 2, however, its amino acid sequence and chromosomal localization are identical to FGF17.