CD178 (soluble) Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL
• Preparation: Produced from sera of goats immunized with highly pure Recombinant Human sFas Ligand
• Anti-Human sFas Ligand-specific antibody was purified by affinity chromatography employing an immobilized Human sFas Ligand matrix
• Sandwich ELISA: To detect Human sFas Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sFas Ligand (500-P184GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sFas Ligand
• Western Blot: To detect Human sFas Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sFasLigand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space
• Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family
• As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR
• CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD)
• The soluble protein can be released from cells upon cleavage by metalloproteinases
• Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins
• CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye
• CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney
• It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses
• CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis
• Various cells express FAS, where CD178 is expressed predominantly on activated T cells
• FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity
• CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension
• The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.