Adipolean variant Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: PGAEGPRGFP GIQGRKGEPG EGAYVYRSAF SVGLETYVTI PNMPIRFTKI FYNQQNHYDG STGKFHCNIP GLYYFAYHIT VYMKDVKVSL FKKDKAMLFT YDQYQENNVD QASGSVLLHL EVGDQVWLQV YGEGERNGLY ADNDNDSTFT GFLLYHDTN
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human gAcrp30/Adipolean Variant
• Anti-Human gAcrp30/Adipolean Variant specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect hAdipolean Variant by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Adipolean Variant (500-P205) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hAdipolean Variant
• Western Blot: To detect Human Adipolean Variant by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adipolean is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P205BT-1MG will be provided as 2 x 500 μg The gAcrp30 variant is a naturally occurring globular protein obtained by proteolytic processing of adiponectin
• Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein
• Like Adiponectin, Acrp30 is capable of decreasing hyperglycemia and reversing insulin resistance
• Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs)
• The signaling receptors for adiponectin and gAcrp30 have recently been identified and named AdipoR1 and AdipoR2
• AdipoR2 is predominantly expressed in the liver
• This naturally occurring variant of human gAcrp30/Adipolean is an 18.1 kDa protein, containing 14 extra amino acids extra at the N-terminus of human gAcrp30/Adipolean.