IL-1 alpha Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-1alpha
• Anti-Human IL-1alpha-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect hIL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-1alpha (500-P21A) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-1alpha
• Western Blot: To detect hIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P21ABT-1MG will be provided as 2 x 500 μg IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells
• IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta
• IL1A is located on the q arm on chromosome 2 at position 13
• IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses
• IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition
• IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis
• Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis
• The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2
• IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases
• IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO)
• High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.