IL-4 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-4
• Anti-Human IL-4-specific antibody was purified by affinity chromatography employing an immobilized Human IL-4 matrix
• Sandwich ELISA: To detect Human IL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-4 (500-P24Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-4
• Western Blot: To detect Human IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor
• IL-4 has been found to have the broadest range of actions
• The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13
• STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4
• The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q
• In particular, IL-4 is located in close proximity to IL13 on the chromosome
• Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome
• IL-4 delta 2 is a known splice variant of IL-4.