IL-21 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-21
• Anti-Murine IL-21-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-21 matrix
• Sandwich ELISA: To detect Murine IL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-21 (500-P278Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-21
• Western Blot: To detect Murine IL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-21 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells
• In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6
• As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function
• IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells
• In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23
• While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3
• IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules
• In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.