IL-9 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MQGCPTLAGI LDINFLINKM QEDPASKCHC SANVTSCLCL GIPSDNCTRP CFSERLSQMT NTTMQTRYPL IFSRVKKSVE VLKNNKCPYF SCEQPCNQTT AGNALTFLKS LLEIFQKEKM RGMRGKI
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-9
• Anti-Human IL-9-specific antibody was purified by affinity chromatography employing an immobilized Human IL-9 matrix
• Sandwich ELISA: To detect Human IL-9 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-9 (500-P29Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-9
• Western Blot: To detect Human IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2μg/mL
• When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes
• IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells
• The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa
• Native mouse IL-9 is heavily glycosylated
• Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.