IFN gamma Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-gamma
• Anti-Human IFN-gamma-specific antibody was purified by affinity chromatography employing an immobilized Human IFN-gamma matrix
• Sandwich ELISA: To detect Human IFN-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IFN-gamma (500-P32Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IFN-gamma
• Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL
• When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines
• IFN gamma is a dimeric protein consisting of two 146 amino acid subunits
• IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa
• On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation
• The biological activity of the IFN gamma homodimer is highly species specific
• Human IFN gamma does not show cross-reactivity with mouse
• IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes
• IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines
• Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway
• IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma
• IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN
• IFN gamma gene mutations are associated with aplastic anemia.