EGF Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EGF
• Anti-Human EGF-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EGF (500-P45) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEGF
• Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P45BT-1MG will be provided as 2 x 500 μg EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase
• Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members
• Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins
• EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands
• EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades
• EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules
• Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade
• Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.