GDNF Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GDNF
• Anti-Human GDNF-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect hGDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GDNF (500-P81) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGDNF
• Western Blot: To detect hGDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P81BT-1MG will be provided as 2 x 500 μg GDNF is a glycosylated, disulfide-bonded homodimer molecule
• It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease
• GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins
• GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.