5061514

ARNT/HIF-1 beta Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 57 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

ARNT/HIF-1 beta

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate

Unconjugated

Host Species

Rabbit

Research Discipline

Autophagy, Cancer, Chromatin Research, HIF Target Genes, Hypoxia, Transcription Factors and Regulators

Formulation

Whole antisera with 0.02% Sodium Azide

Gene Alias

ARNT protein, aryl hydrocarbon receptor nuclear translocator, BHLHE2, HIF-1 Beta, HIF1B, HIF-1-beta, HIF1-beta, HIF1BETA, hypoxia-inducible factor 1, beta subunit, Hypoxia-inducible factor 1-beta, nuclear translocator

Gene Symbols

ARNT

Isotype

IgG

Purification Method

Unpurified

Test Specificity

This is specific for HIF-1 beta/ARNT. It is not known if NB100-110 cross-reacts with ARNT2 which is related to HIF-1 beta/ARNT but is the product of a different gene.

Dilution

Western Blot 1:2000, Simple Western 1:1000, Immunohistochemistry 1:150, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:150, Immunoblotting, Gel Supershift Assay, Chromatin Immunoprecipitation (ChIP) 1:10-1:500

Classification

Polyclonal

Form

Antisera

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Bovine, Canine, Fish, Ferret, Sheep

Gene Accession No.

P27540

Gene ID (Entrez)

405

Immunogen

Fusion protein to human HIF-1 beta containing amino acids 496-789. [UniProt# P27540]

Primary or Secondary

Primary

Content And Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

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Description

  • ARNT/HIF-1 beta Polyclonal specifically detects ARNT/HIF-1 beta in Human, Mouse, Rat, Bovine, Canine, Fish, Ferret, Sheep samples
  • It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunoblotting, Gel Supershift Assay, Chromatin Immunoprecipitation (ChIP).

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Img

Fischer Scientific

5061514

Rabbit Polyclonal Antibody has been...


Antigen:
ARNT/HIF-1 beta

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Autophagy, Cancer, Chromatin Research, HIF Target Genes, Hypoxia, Transcription Factors and Regulators

Formulation:
Whole antisera with 0.02% Sodium Azide

Gene Alias:
ARNT protein, aryl hydrocarbon receptor nuclear translocator, BHLHE2, HIF-1 Beta, HIF1B, HIF-1-beta, HIF1-beta, HIF1BETA, hypoxia-inducible factor 1, beta subunit, Hypoxia-inducible factor 1-beta, nuclear translocator

Gene Symbols:
ARNT

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
This is specific for HIF-1 beta/ARNT. It is not known if NB100-110 cross-reacts with ARNT2 which is related to HIF-1 beta/ARNT but is the product of a different gene.

Dilution:
Western Blot 1:2000, Simple Western 1:1000, Immunohistochemistry 1:150, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:150, Immunoblotting, Gel Supershift Assay, Chromatin Immunoprecipitation (ChIP) 1:10-1:500

Classification:
Polyclonal

Form:
Antisera

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Bovine, Canine, Fish, Ferret, Sheep

Gene Accession No.:
P27540

Gene ID (Entrez):
405

Immunogen:
Fusion protein to human HIF-1 beta containing amino acids 496-789. [UniProt# P27540]

Primary or Secondary:
Primary

Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Img

Fischer Scientific

5061565

Rabbit Polyclonal Antibody has been...


Antigen:
Aromatase

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Breast Cancer, Cancer, Cell Biology, Cell Cycle and Replication, Chromatin Research, Lipid and Metabolism, Neuroscience

Formulation:
PBS with 0.02% Sodium Azide

Gene Alias:
ARO1EC 1.14.14.1, Aromatase, AROsubfamily XIX (aromatization of androgens), CYARMGC104309, CYPXIX, cytochrome P450, family 19, subfamily A, polypeptide 1, Cytochrome P-450AROM, Estrogen synthase, microsomal monooxygenase

Gene Symbols:
CYP19A1

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Human and Primate. Immunogen has 91% homology to horse, 90% homology to cow and rabbit, 83% homology to pig, and 81% homology to sheep, goat, and dog proteins.

Dilution:
Western Blot 1 ug/ml, Simple Western reported in scientific literature (PMID 29898754), Immunohistochemistry 1:200. Use reported in scientific literature (PMID 28133606), Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:200

Classification:
Polyclonal

Form:
__

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Primate, Rabbit

Gene Accession No.:
P11511

Gene ID (Entrez):
1588

Immunogen:
A synthetic peptide made to a C-terminal portion of the human Aromatase protein (between residues 400-502). [UniProt# P11511]

Primary or Secondary:
Primary

Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Img

Fischer Scientific

5061565

Rabbit Polyclonal Antibody has been...


Antigen:
Aromatase

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Breast Cancer, Cancer, Cell Biology, Cell Cycle and Replication, Chromatin Research, Lipid and Metabolism, Neuroscience

Formulation:
PBS with 0.02% Sodium Azide

Gene Alias:
ARO1EC 1.14.14.1, Aromatase, AROsubfamily XIX (aromatization of androgens), CYARMGC104309, CYPXIX, cytochrome P450, family 19, subfamily A, polypeptide 1, Cytochrome P-450AROM, Estrogen synthase, microsomal monooxygenase

Gene Symbols:
CYP19A1

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Human and Primate. Immunogen has 91% homology to horse, 90% homology to cow and rabbit, 83% homology to pig, and 81% homology to sheep, goat, and dog proteins.

Dilution:
Western Blot 1 ug/ml, Simple Western reported in scientific literature (PMID 29898754), Immunohistochemistry 1:200. Use reported in scientific literature (PMID 28133606), Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:200

Classification:
Polyclonal

Form:
__

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Primate, Rabbit

Gene Accession No.:
P11511

Gene ID (Entrez):
1588

Immunogen:
A synthetic peptide made to a C-terminal portion of the human Aromatase protein (between residues 400-502). [UniProt# P11511]

Primary or Secondary:
Primary

Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

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Fischer Scientific

5061578

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Antigen:
CYP1B1

Applications:
Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), In vitro Assay

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Cancer, Cardiovascular Biology, Cellular Signaling, metabolism

Formulation:
PBS with 0.05% Sodium Azide

Gene Alias:
aryl hydrocarbon hydroxylase, CP1B, CYPIB1, cytochrome P450 1B1, cytochrome P450, family 1, subfamily B, polypeptide 1, cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, EC 1.14.14.1, flavoprotein-linked monooxygenase, GLC3A, microsomal monooxygenase, P4501B1, primary infantile), xenobiotic monooxygenase

Gene Symbols:
CYP1B1

Isotype:
IgG

Purification Method:
Protein G purified

Test Specificity:
The amino acid sequence used as immunogen is 100% homologous in human, 93% in bovine and pig, and 87% in mouse, and dog.

Dilution:
Western Blot 0.5 to 2 μg/mL, Immunohistochemistry, Immunohistochemistry-Paraffin 5 to 10 μg/mL, In vitro assay

Classification:
Polyclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
__

Gene ID (Entrez):
1545

Immunogen:
A portion of amino acids 400-450 of human CYP1B1 was used as the immunogen.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.