5068066

Enolase 1 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 4 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Enolase 1

Dilution

Western Blot 1:100-1:2000, Simple Western 1:5000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:25-1:100, Immunohistochemistry-Frozen 1:25-1:100

Classification

Polyclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse, Bovine

Gene Accession No.

P06733

Gene ID (Entrez)

2023

Immunogen

Native human Enolase 1

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Concentration

5 mg/mL

Applications

Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Rabbit

Research Discipline

Cancer, Core ESC Like Genes, Stem Cell Markers

Formulation

Phosphate buffered saline with 0.09% Sodium Azide

Gene Alias

2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin

Gene Symbols

ENO1

Isotype

IgG

Purification Method

Protein G purified

Test Specificity

This recognizes human Non-neuronal enolase, and demonstrates less than 0.1% cross-reactivity with non-specific enolase. Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. The gene coding for the 48kDa form of non-neuronal enolase also codes for a shorter 37kDa isoform, myc-binding protein 1 (MBP-1) lacking the N-terminal 93 amino acids of non-neuronal enolase.

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Description

  • Enolase 1 Polyclonal specifically detects Enolase 1 in Human, Mouse, Bovine samples
  • It is validated for Western Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

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Img

Fischer Scientific

5068066

Rabbit Polyclonal Antibody has been...


Antigen:
Enolase 1

Dilution:
Western Blot 1:100-1:2000, Simple Western 1:5000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:25-1:100, Immunohistochemistry-Frozen 1:25-1:100

Classification:
Polyclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Bovine

Gene Accession No.:
P06733

Gene ID (Entrez):
2023

Immunogen:
Native human Enolase 1

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Concentration:
5 mg/mL

Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Cancer, Core ESC Like Genes, Stem Cell Markers

Formulation:
Phosphate buffered saline with 0.09% Sodium Azide

Gene Alias:
2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin

Gene Symbols:
ENO1

Isotype:
IgG

Purification Method:
Protein G purified

Test Specificity:
This recognizes human Non-neuronal enolase, and demonstrates less than 0.1% cross-reactivity with non-specific enolase. Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. The gene coding for the 48kDa form of non-neuronal enolase also codes for a shorter 37kDa isoform, myc-binding protein 1 (MBP-1) lacking the N-terminal 93 amino acids of non-neuronal enolase.

Img

Fischer Scientific

5068078

Rabbit Polyclonal Antibody has been...


Antigen:
Enolase 2/Neuron-specific Enolase

Dilution:
Western Blot 1:2000, Simple Western 1:10000, Immunohistochemistry 1:500, Immunocytochemistry/Immunofluorescence 1:500, Knockdown Validated

Classification:
Polyclonal

Form:
Antisera

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat

Gene Accession No.:
P09104

Gene ID (Entrez):
2026

Immunogen:
Recombinant full length human NSE purified from E. coli. [UniProt# P09104]

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Concentration:
__

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, KnockDown

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Cancer, Cellular Markers, Neuronal Cell Markers, Neuroscience

Formulation:
Supplied as serum with 5mM Sodium Azide

Gene Alias:
2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, Enolase 2, enolase 2 (gamma, neuronal), gamma-enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuron-specific enolase, NSE

Gene Symbols:
ENO2

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
Mouse data from customer review.

Img

Fischer Scientific

5068078

Rabbit Polyclonal Antibody has been...


Antigen:
Enolase 2/Neuron-specific Enolase

Dilution:
Western Blot 1:2000, Simple Western 1:10000, Immunohistochemistry 1:500, Immunocytochemistry/Immunofluorescence 1:500, Knockdown Validated

Classification:
Polyclonal

Form:
Antisera

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat

Gene Accession No.:
P09104

Gene ID (Entrez):
2026

Immunogen:
Recombinant full length human NSE purified from E. coli. [UniProt# P09104]

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Concentration:
__

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, KnockDown

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Cancer, Cellular Markers, Neuronal Cell Markers, Neuroscience

Formulation:
Supplied as serum with 5mM Sodium Azide

Gene Alias:
2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, Enolase 2, enolase 2 (gamma, neuronal), gamma-enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuron-specific enolase, NSE

Gene Symbols:
ENO2

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
Mouse data from customer review.

Img

Fischer Scientific

5068081

Rabbit Polyclonal Antibody has been...


Antigen:
GLP/EHMT1

Dilution:
Western Blot reported in scientific literature (PMID 30858340), Immunohistochemistry 1:150, Immunocytochemistry/Immunofluorescence 1:50-1:100, Immunohistochemistry-Paraffin 1:150, KnockDown Validated reported in scientific literature (PMID 30858340)

Classification:
Polyclonal

Form:
__

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
Q9H9B1

Gene ID (Entrez):
79813

Immunogen:
A genomic peptide made to an N-terminal region of the human EHMT1 protein (within residues 20-200). [Swiss-Prot Q9H9B1]

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Concentration:
0.4 mg/mL

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), KnockDown

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Breast Cancer

Formulation:
PBS and 30% Glycerol with 0.05% Sodium Azide

Gene Alias:
EC 2.1.1.-, EC 2.1.1.43, euchromatic histone-lysine N-methyltransferase 1H3-K9-HMTase 5, Eu-HMTase1DEL9q34, EUHMTASE1FP13812, FLJ12879, G9a-like protein 1, GLP, GLP1, H3 lysine-9 specific 5, KIAA1876euchromatic histone methyltransferase 1, KMT1DDKFZp667M072

Gene Symbols:
EHMT1

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
__