5073500

HMGB1/HMG-1 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 22 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

HMGB1/HMG-1

Dilution

Western Blot 0.5-1.0 ug/ml, Simple Western 1:2000, Flow Cytometry 1:100, Immunohistochemistry 1:100-1:250, Immunocytochemistry/Immunofluorescence 0.05 ug/ml, Immunohistochemistry-Paraffin 1:100-1:250, Flow (Intracellular)

Classification

Polyclonal

Host Species

Rabbit

Research Discipline

Cellular Markers, Chromatin Research, Histones and Modified Histones, Neuroscience, Transcription Factors and Regulators

Formulation

Tris-Glycine with 0.02% Sodium Azide

Gene Alias

Amphoterin, high mobility group box 1, High mobility group protein 1, high mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydrate binding protein

Gene Symbols

HMGB1

Isotype

IgG

Purification Method

Affinity Purified

Test Specificity

Human, mouse, rat, dog, bovine, sheep. Predicted to react with porcine, equine and rabbit based on 100% sequence homology.

Concentration

1 mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Bovine, Canine, Rabbit, Sheep

Gene Accession No.

P09429

Gene ID (Entrez)

3146

Immunogen

A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]

Primary or Secondary

Primary

Content And Storage

Store at 4C. Do not freeze.

Molecular Weight of Antigen

29 kDa

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Description

  • HMGB1/HMG-1 Polyclonal specifically detects HMGB1/HMG-1 in Human, Mouse, Rat, Bovine, Canine, Rabbit, Sheep samples
  • It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular), Knockdown Validated.

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Antigen:
HMGB1/HMG-1

Dilution:
Western Blot 0.5-1.0 ug/ml, Simple Western 1:2000, Flow Cytometry 1:100, Immunohistochemistry 1:100-1:250, Immunocytochemistry/Immunofluorescence 0.05 ug/ml, Immunohistochemistry-Paraffin 1:100-1:250, Flow (Intracellular)

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cellular Markers, Chromatin Research, Histones and Modified Histones, Neuroscience, Transcription Factors and Regulators

Formulation:
Tris-Glycine with 0.02% Sodium Azide

Gene Alias:
Amphoterin, high mobility group box 1, High mobility group protein 1, high mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydrate binding protein

Gene Symbols:
HMGB1

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Human, mouse, rat, dog, bovine, sheep. Predicted to react with porcine, equine and rabbit based on 100% sequence homology.

Concentration:
1 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Bovine, Canine, Rabbit, Sheep

Gene Accession No.:
P09429

Gene ID (Entrez):
3146

Immunogen:
A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]

Primary or Secondary:
Primary

Content And Storage:
Store at 4C. Do not freeze.

Molecular Weight of Antigen:
29 kDa

Img

Fischer Scientific

5073502

Rabbit Polyclonal Antibody...


Antigen:
HMGB1/HMG-1

Dilution:
Western Blot 5-10ug/ml∼, Simple Western 1:100, Flow Cytometry 0.5ug/10^6cells, Immunohistochemistry-Paraffin 5ug/ml

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cellular Markers, Chromatin Research, Histones and Modified Histones, Neuroscience, Transcription Factors and Regulators

Formulation:
0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
Amphoterin, high mobility group box 1, High mobility group protein 1, high mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydrate binding protein

Gene Symbols:
HMGB1

Isotype:
IgG

Purification Method:
Protein A purified

Test Specificity:
The HMGB1 amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, chicken, cow and monkey. This antibody recognizes both B box domain (amino acids 88-162) and full length HMGB1 protein.

Concentration:
0.5 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
P09429

Gene ID (Entrez):
3146

Immunogen:
A portion of amino acids 100-166 of human HMGB1 was used as the immunogen.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__

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5073506

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Antigen:
HMGB3/HMG4

Dilution:
Western Blot 0.5-1.0 ug/ml

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Chromatin Research, DNA Repair

Formulation:
0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
High mobility group protein 2a, High mobility group protein 4, high mobility group protein B3, high-mobility group (nonhistone chromosomal) protein 4, high-mobility group box 3, HMG-2a, HMG2AHMG-4, HMG4MGC90319, non-histone chromosomal protein

Gene Symbols:
HMGB3

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_c).

Concentration:
0.5 mg/mL

Applications:
Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Bovine, Canine, Chicken, Equine, Primate

Gene Accession No.:
O15347

Gene ID (Entrez):
3149

Immunogen:
A portion of amino acids 150-200 of human HMGB3 was used as the immunogen.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__

Img

Fischer Scientific

5073507

Rabbit Polyclonal Antibody...


Antigen:
HMGB3/HMG4

Dilution:
Western Blot 3-5 ug/ml

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Chromatin Research, DNA Repair

Formulation:
0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
High mobility group protein 2a, High mobility group protein 4, high mobility group protein B3, high-mobility group (nonhistone chromosomal) protein 4, high-mobility group box 3, HMG-2a, HMG2AHMG-4, HMG4MGC90319, non-histone chromosomal protein

Gene Symbols:
HMGB3

Isotype:
IgG

Purification Method:
Protein A purified

Test Specificity:
The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, chimpanzee, rhesus monkey and dog, 96% homologous in cow, 93% homologous inopossumand 80% homologous in chicken.

Concentration:
0.5 mg/mL

Applications:
Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Bovine, Equine, Opossum

Gene Accession No.:
O15347

Gene ID (Entrez):
3149

Immunogen:
A portion of amino acids 1-50 of human HMGB3 was used as the immunogen.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__