5077144
PKM1 Antibody - BSA Free, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 14 publications
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
PKM1
Dilution
Western Blot 0.5 ug/ml, Simple Western 1:400, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence reported in multiple pieces of scientific literature, Immunohistochemistry-Paraffin 1:400, Immunoblotting reported in scientific literature (PMID 25416385)
Classification
Polyclonal
Host Species
Rabbit
Target Species
Human, Mouse, Bovine
Gene Accession No.
P14618, P52480
Gene ID (Entrez)
5315
Immunogen
A synthetic peptide made to a C-terminal portion of the human PKM1 protein (between residues 350-450) [UniProt P14618-2].
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration
1.17 mg/mL
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Regulatory Status
RUO
Formulation
PBS and 30% Glycerol with 0.05% Sodium Azide
Gene Alias
PK1, Pyruvate kinase isozyme M1, Pyruvate kinase isozymes M1, Pyruvate kinase M1
Gene Symbols
PKM
Isotype
IgG
Purification Method
Affinity Purified
Test Specificity
This antibody is specific for the M1 isoform of PKM.
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Description
- PKM1 Polyclonal specifically detects PKM1 in Human, Mouse, Bovine samples
- It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoblotting.
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Antigen: PKM1
Dilution: Western Blot 0.5 ug/ml, Simple Western 1:400, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence reported in multiple pieces of scientific literature, Immunohistochemistry-Paraffin 1:400, Immunoblotting reported in scientific literature (PMID 25416385)
Classification: Polyclonal
Host Species: Rabbit
Target Species: Human, Mouse, Bovine
Gene Accession No.: P14618, P52480
Gene ID (Entrez): 5315
Immunogen: A synthetic peptide made to a C-terminal portion of the human PKM1 protein (between residues 350-450) [UniProt P14618-2].
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: 1.17 mg/mL
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: PBS and 30% Glycerol with 0.05% Sodium Azide
Gene Alias: PK1, Pyruvate kinase isozyme M1, Pyruvate kinase isozymes M1, Pyruvate kinase M1
Gene Symbols: PKM
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This antibody is specific for the M1 isoform of PKM.
Antigen:
PKM1
Dilution:
Western Blot 0.5 ug/ml, Simple Western 1:400, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence reported in multiple pieces of scientific literature, Immunohistochemistry-Paraffin 1:400, Immunoblotting reported in scientific literature (PMID 25416385)
Classification:
Polyclonal
Host Species:
Rabbit
Target Species:
Human, Mouse, Bovine
Gene Accession No.:
P14618, P52480
Gene ID (Entrez):
5315
Immunogen:
A synthetic peptide made to a C-terminal portion of the human PKM1 protein (between residues 350-450) [UniProt P14618-2].
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
1.17 mg/mL
Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
PBS and 30% Glycerol with 0.05% Sodium Azide
Gene Alias:
PK1, Pyruvate kinase isozyme M1, Pyruvate kinase isozymes M1, Pyruvate kinase M1
Gene Symbols:
PKM
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
This antibody is specific for the M1 isoform of PKM.
Antigen: Ku80/XRCC5
Dilution: Western Blot 1:500, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Classification: Polyclonal
Host Species: Rabbit
Target Species: Human, Mouse, Rat, Hamster
Gene Accession No.: __
Gene ID (Entrez): 7520
Immunogen: A synthetic peptide corresponding to residues 419-440 LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: 1 mg/mL
Applications: Western Blot, Immunoprecipitation, ChIP Assay
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: PBS with 0.02% Sodium Azide
Gene Alias: 80kDa, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC85, CTCBF, EC 3.6.4.-, FLJ39089, G22P2, KARP1, KARP-1, KU80, Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen, Ku86DNA repair protein XRCC5, KUB2, NFIV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit, X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD), X-ray repair cross-complementing protein 5, X-ray repair, complementing defective, repair in Chinese hamster
Gene Symbols: XRCC5
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: Reacts with residues 419-440 [LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse, rat and hamster.
Antigen:
Ku80/XRCC5
Dilution:
Western Blot 1:500, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Classification:
Polyclonal
Host Species:
Rabbit
Target Species:
Human, Mouse, Rat, Hamster
Gene Accession No.:
__
Gene ID (Entrez):
7520
Immunogen:
A synthetic peptide corresponding to residues 419-440 LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
1 mg/mL
Applications:
Western Blot, Immunoprecipitation, ChIP Assay
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
PBS with 0.02% Sodium Azide
Gene Alias:
80kDa, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC85, CTCBF, EC 3.6.4.-, FLJ39089, G22P2, KARP1, KARP-1, KU80, Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen, Ku86DNA repair protein XRCC5, KUB2, NFIV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit, X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD), X-ray repair cross-complementing protein 5, X-ray repair, complementing defective, repair in Chinese hamster
Gene Symbols:
XRCC5
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
Reacts with residues 419-440 [LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse, rat and hamster.
Antigen: Park7/DJ-1
Dilution: Western Blot 1:2000, Simple Western 1:4000, Flow Cytometry reported in scientific literature (PMID 21937704), Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:500, Immunoprecipitation 1:200, Immunohistochemistry-Paraffin, In vitro assay reported in scientific literature (PMID 21645620)
Classification: Polyclonal
Host Species: Rabbit
Target Species: Human, Mouse, Rat, Chicken
Gene Accession No.: Q99497
Gene ID (Entrez): 11315
Immunogen: A synthetic peptide corresponding to the C-terminus (within residues 150-189) of human Park7(DJ-1). [UniProt# Q99497]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: __
Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, In vitro Assay
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Whole antisera with No Preservative
Gene Alias: DJ-1, DJ1FLJ34360, EC 3.4, FLJ27376, FLJ92274, Oncogene DJ1, Parkinson disease (autosomal recessive, early onset) 7, Parkinson disease protein 7, protein DJ-1
Gene Symbols: PARK7
Isotype: IgG
Purification Method: Unpurified
Test Specificity: Human and mouse. Predicted to react with rat, bovine, Zebra fish, Golden Syrian hamster, and chicken based on 100% sequence homology. Chicken reactivity reported in scientific literature (PMID: 24064392).
Antigen:
Park7/DJ-1
Dilution:
Western Blot 1:2000, Simple Western 1:4000, Flow Cytometry reported in scientific literature (PMID 21937704), Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:500, Immunoprecipitation 1:200, Immunohistochemistry-Paraffin, In vitro assay reported in scientific literature (PMID 21645620)
Classification:
Polyclonal
Host Species:
Rabbit
Target Species:
Human, Mouse, Rat, Chicken
Gene Accession No.:
Q99497
Gene ID (Entrez):
11315
Immunogen:
A synthetic peptide corresponding to the C-terminus (within residues 150-189) of human Park7(DJ-1). [UniProt# Q99497]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
__
Applications:
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, In vitro Assay
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Whole antisera with No Preservative
Gene Alias:
DJ-1, DJ1FLJ34360, EC 3.4, FLJ27376, FLJ92274, Oncogene DJ1, Parkinson disease (autosomal recessive, early onset) 7, Parkinson disease protein 7, protein DJ-1
Gene Symbols:
PARK7
Isotype:
IgG
Purification Method:
Unpurified
Test Specificity:
Human and mouse. Predicted to react with rat, bovine, Zebra fish, Golden Syrian hamster, and chicken based on 100% sequence homology. Chicken reactivity reported in scientific literature (PMID: 24064392).
Antigen: Patched 1/PTCH
Dilution: Western Blot 1:2500, Immunohistochemistry 1:50 - 1:250, Immunohistochemistry-Paraffin 1:50 - 1:250
Classification: Polyclonal
Host Species: Rabbit
Target Species: Human, Mouse
Gene Accession No.: Q13635
Gene ID (Entrez): 5727
Immunogen: A genomic peptide made to a C-terminal region of the human Patched 1 protein (within residues 1200-1350). [Swiss-Prot Q13635]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: 1.22 mg/mL
Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: PBS with 0.05% Sodium Azide
Gene Alias: BCNSFLJ42602, FLJ26746, HPE7, NBCCS, patched (Drosophila) homolog, patched 1, patched homolog (Drosophila), patched homolog 1, patched homolog 1 (Drosophila), protein patched homolog 1, PTC, PTC1, PTCH, PTCH protein +12b, PTCH protein +4', PTCH protein -10, PTCH11
Gene Symbols: PTCH1
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: __
Antigen:
Patched 1/PTCH
Dilution:
Western Blot 1:2500, Immunohistochemistry 1:50 - 1:250, Immunohistochemistry-Paraffin 1:50 - 1:250
Classification:
Polyclonal
Host Species:
Rabbit
Target Species:
Human, Mouse
Gene Accession No.:
Q13635
Gene ID (Entrez):
5727
Immunogen:
A genomic peptide made to a C-terminal region of the human Patched 1 protein (within residues 1200-1350). [Swiss-Prot Q13635]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
1.22 mg/mL
Applications:
Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
PBS with 0.05% Sodium Azide
Gene Alias:
BCNSFLJ42602, FLJ26746, HPE7, NBCCS, patched (Drosophila) homolog, patched 1, patched homolog (Drosophila), patched homolog 1, patched homolog 1 (Drosophila), protein patched homolog 1, PTC, PTC1, PTCH, PTCH protein +12b, PTCH protein +4', PTCH protein -10, PTCH11
Gene Symbols:
PTCH1
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
__