5082041
PU.1/Spi-1 Antibody, Novus Biologicals™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
PU.1/Spi-1
Dilution
Western Blot 4-6ug/ml, Flow Cytometry 1.25 ug/10^6 cells
Classification
Polyclonal
Form
Purified
Regulatory Status
RUO
Formulation
0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias
hematopoietic transcription factor PU.1, OF, PU.1, SFPI131 kDa transforming protein, SPI-1, SPI-1 proto-oncogene, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein, transcription factor PU.1
Gene Symbols
SPI1
Isotype
IgG
Purification Method
Protein A purified
Test Specificity
The amino acid sequence used as immunogen is 100% homologous in human, mouse, chimpanzee, monkey, dog, panda, horse, cow, and wild pig, 92% homologous in rat, 89% homologous in opossum,77% homologous in zebra finch and clearnose skate, and 71% homologous in chicken and dwarf caiman.
Concentration
0.5 mg/mL
Applications
Western Blot, Flow Cytometry
Conjugate
Unconjugated
Host Species
Rabbit
Target Species
Human, Mouse, Bovine, Canine, Equine, Primate
Gene Accession No.
P17947
Gene ID (Entrez)
6688
Immunogen
A portion of amino acid 20-70 of human PU.1 was used as the immunogen.
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Related Products
Description
- Description PU.1/Spi-1 Polyclonal specifically detects PU.1/Spi-1 in Human, Mouse, Bovine, Canine, Equine, Primate samples
- It is validated for Western Blot, Flow Cytometry.
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Antigen: PU.1/Spi-1
Dilution: Western Blot 4-6ug/ml, Flow Cytometry 1.25 ug/10^6 cells
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: 0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: hematopoietic transcription factor PU.1, OF, PU.1, SFPI131 kDa transforming protein, SPI-1, SPI-1 proto-oncogene, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein, transcription factor PU.1
Gene Symbols: SPI1
Isotype: IgG
Purification Method: Protein A purified
Test Specificity: The amino acid sequence used as immunogen is 100% homologous in human, mouse, chimpanzee, monkey, dog, panda, horse, cow, and wild pig, 92% homologous in rat, 89% homologous in opossum,77% homologous in zebra finch and clearnose skate, and 71% homologous in chicken and dwarf caiman.
Concentration: 0.5 mg/mL
Applications: Western Blot, Flow Cytometry
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse, Bovine, Canine, Equine, Primate
Gene Accession No.: P17947
Gene ID (Entrez): 6688
Immunogen: A portion of amino acid 20-70 of human PU.1 was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
PU.1/Spi-1
Dilution:
Western Blot 4-6ug/ml, Flow Cytometry 1.25 ug/10^6 cells
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
hematopoietic transcription factor PU.1, OF, PU.1, SFPI131 kDa transforming protein, SPI-1, SPI-1 proto-oncogene, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein, transcription factor PU.1
Gene Symbols:
SPI1
Isotype:
IgG
Purification Method:
Protein A purified
Test Specificity:
The amino acid sequence used as immunogen is 100% homologous in human, mouse, chimpanzee, monkey, dog, panda, horse, cow, and wild pig, 92% homologous in rat, 89% homologous in opossum,77% homologous in zebra finch and clearnose skate, and 71% homologous in chicken and dwarf caiman.
Concentration:
0.5 mg/mL
Applications:
Western Blot, Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse, Bovine, Canine, Equine, Primate
Gene Accession No.:
P17947
Gene ID (Entrez):
6688
Immunogen:
A portion of amino acid 20-70 of human PU.1 was used as the immunogen.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: MyoD
Dilution: Western Blot 1:5000, Immunocytochemistry/Immunofluorescence 1:100
Classification: Polyclonal
Form: __
Regulatory Status: RUO
Formulation: PBS, 0.1% BSA, and 50% Glycerol with 0.05% Sodium Azide
Gene Alias: bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
Gene Symbols: MYOD1
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: Human. Immunogen has 90% identity to mouse.
Concentration: 0.58 mg/mL
Applications: Western Blot, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse
Gene Accession No.: P15172, P10085
Gene ID (Entrez): 4654
Immunogen: A partial recombinant protein made to an N-terminal region of the human MyoD1 protein (within residues 1-150). [Swiss-Prot# P15172]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
MyoD
Dilution:
Western Blot 1:5000, Immunocytochemistry/Immunofluorescence 1:100
Classification:
Polyclonal
Form:
__
Regulatory Status:
RUO
Formulation:
PBS, 0.1% BSA, and 50% Glycerol with 0.05% Sodium Azide
Gene Alias:
bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
Gene Symbols:
MYOD1
Isotype:
IgG
Purification Method:
Affinity Purified
Test Specificity:
Human. Immunogen has 90% identity to mouse.
Concentration:
0.58 mg/mL
Applications:
Western Blot, Immunocytochemistry, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse
Gene Accession No.:
P15172, P10085
Gene ID (Entrez):
4654
Immunogen:
A partial recombinant protein made to an N-terminal region of the human MyoD1 protein (within residues 1-150). [Swiss-Prot# P15172]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: PUMA
Dilution: Western Blot 2-5 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS containing 0.05% BSA with 0.05% Sodium Azide
Gene Alias: BCL2 binding component 3, bcl-2-binding component 3, JFY1, JFY-1, p53 up-regulated modulator of apoptosis, PUMAFLJ42994
Gene Symbols: BBC3
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: The amino acid sequence used as immunogen has 100% identity in PUMAd and PUMAg, and 59% identity in PUMAa. For additional information on PUMA isoforms and their sequences, see Nakano et al. 2001.
Concentration: 0.5 mg/mL
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse
Gene Accession No.: NP_001120712.1
Gene ID (Entrez): 27113
Immunogen: A synthetic peptide (AAPPLTLEGPVQSHHGTP) corresponding to amino acids 36-53 of human PUMA d (GenBank no. gb AAK39544.1 AF354656_1 ) and amino acids 196-213 of human PUMA g (GenBank no ref NP_001120712.1 ) was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
PUMA
Dilution:
Western Blot 2-5 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS containing 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
BCL2 binding component 3, bcl-2-binding component 3, JFY1, JFY-1, p53 up-regulated modulator of apoptosis, PUMAFLJ42994
Gene Symbols:
BBC3
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
The amino acid sequence used as immunogen has 100% identity in PUMAd and PUMAg, and 59% identity in PUMAa. For additional information on PUMA isoforms and their sequences, see Nakano et al. 2001.
Concentration:
0.5 mg/mL
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse
Gene Accession No.:
NP_001120712.1
Gene ID (Entrez):
27113
Immunogen:
A synthetic peptide (AAPPLTLEGPVQSHHGTP) corresponding to amino acids 36-53 of human PUMA d (GenBank no. gb AAK39544.1 AF354656_1 ) and amino acids 196-213 of human PUMA g (GenBank no ref NP_001120712.1 ) was used as the immunogen.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: MafA
Dilution: Western Blot 0.25-0.5ug/ml, Chromatin Immunoprecipitation reported in scientific literature (PMID 24509877), Immunohistochemistry, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 24509877), Immunohistochemistry-Paraffin reported in scientific literature (PMID 24509877), ICC/IF (Negative), Chromatin Immunoprecipitation (ChIP)
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: 0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: hMafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3B1, transcription factor MafA, Transcription factor RIPE3b1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)
Gene Symbols: MAFA
Isotype: IgG
Purification Method: Protein A purified
Test Specificity: The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, pig and bovine, 87% homologous in pufferfish and 81% homologous in zebrafish.
Concentration: 0.5 mg/mL
Applications: Western Blot, ChIP Assay, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse, Rat, Opossum
Gene Accession No.: Q8NHW3
Gene ID (Entrez): 389692
Immunogen: A portion of amino acids 300-350 of human MafA was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
MafA
Dilution:
Western Blot 0.25-0.5ug/ml, Chromatin Immunoprecipitation reported in scientific literature (PMID 24509877), Immunohistochemistry, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 24509877), Immunohistochemistry-Paraffin reported in scientific literature (PMID 24509877), ICC/IF (Negative), Chromatin Immunoprecipitation (ChIP)
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
hMafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3B1, transcription factor MafA, Transcription factor RIPE3b1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)
Gene Symbols:
MAFA
Isotype:
IgG
Purification Method:
Protein A purified
Test Specificity:
The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, pig and bovine, 87% homologous in pufferfish and 81% homologous in zebrafish.
Concentration:
0.5 mg/mL
Applications:
Western Blot, ChIP Assay, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse, Rat, Opossum
Gene Accession No.:
Q8NHW3
Gene ID (Entrez):
389692
Immunogen:
A portion of amino acids 300-350 of human MafA was used as the immunogen.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.