SARS Spike Protein Antibody, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 15 publications
Manufacturer: Fischer Scientific
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Antigen
Sars Spike Protein
Dilution
Western Blot 0.05 - 1.0 ug/mL, Simple Western 1:40 - 1:160, Immunohistochemistry reported in scientific literature (PMID 34357881), Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 16081529)
Classification
Polyclonal
Form
Purified
Regulatory Status
RUO
Formulation
PBS with 0.02% Sodium Azide
Gene ID (Entrez)
1489668
Isotype
IgG
Purification Method
Immunogen affinity purified
Test Specificity
Full length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139 kDa band was observed. Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Spike Protein, 1000-1200 a.a. (NBP2-90973) and low/no reactivity towards MERS Spike 2 or H1N1 (NBP1-99041).
Concentration
1.0 mg/ml
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Conjugate
Unconjugated
Host Species
Rabbit
Target Species
SARS-CoV, SARS-CoV-2
Gene Alias
COVID-19 Spike, E2, E2 glycoprotein, Human coronavirus spike glycoprotein, Peplomer protein, S, S glycoprotein, SARS coronavirus S protein, SARS coronavirus Spike Protein, SARS CoV, SARS CoV S glycoprotein, SARS CoV Spike protein, SARSCoV, SARS-CoV S protein, SARS-COV Spike, SARS-COV-2 S protein, SARS-COV-2 Spike glycoprotein, SARSCOV2 Spike protein, Severe acute respiratory syndrome spike glycoprotein, Severe acute respiratory syndrome virus spike glycoprotein, Spike glycoprotein, VGL2
Immunogen
The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 1124-1140 (C-GNCDVVIGIVNNTVYDP) from the S (Spike glycoprotein)(Spike protein S2') for the Human SARS coronavirus (Genbank accession no. YP_009724390)
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Related Products
Description
- SARS Spike Protein Polyclonal specifically detects SARS Spike Protein in SARS-CoV, SARS-CoV-2 samples
- It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.
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Antigen: Sars Spike Protein
Dilution: Western Blot 0.05 - 1.0 ug/mL, Simple Western 1:40 - 1:160, Immunohistochemistry reported in scientific literature (PMID 34357881), Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 16081529)
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.02% Sodium Azide
Gene ID (Entrez): 1489668
Isotype: IgG
Purification Method: Immunogen affinity purified
Test Specificity: Full length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139 kDa band was observed. Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Spike Protein, 1000-1200 a.a. (NBP2-90973) and low/no reactivity towards MERS Spike 2 or H1N1 (NBP1-99041).
Concentration: 1.0 mg/ml
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: SARS-CoV, SARS-CoV-2
Gene Alias: COVID-19 Spike, E2, E2 glycoprotein, Human coronavirus spike glycoprotein, Peplomer protein, S, S glycoprotein, SARS coronavirus S protein, SARS coronavirus Spike Protein, SARS CoV, SARS CoV S glycoprotein, SARS CoV Spike protein, SARSCoV, SARS-CoV S protein, SARS-COV Spike, SARS-COV-2 S protein, SARS-COV-2 Spike glycoprotein, SARSCOV2 Spike protein, Severe acute respiratory syndrome spike glycoprotein, Severe acute respiratory syndrome virus spike glycoprotein, Spike glycoprotein, VGL2
Immunogen: The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 1124-1140 (C-GNCDVVIGIVNNTVYDP) from the S (Spike glycoprotein)(Spike protein S2') for the Human SARS coronavirus (Genbank accession no. YP_009724390)
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: Sars Spike Protein
Dilution: Western Blot 2 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS containing 0.2% gelatin with 0.05% Sodium Azide
Gene ID (Entrez): 1489668
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: SARS-Spike protein
Concentration: 0.5 mg/mL
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: SARS-CoV
Gene Alias: E2, E2 glycoprotein, Human coronavirus spike glycoprotein, Peplomer protein, S, S glycoprotein, SARS coronavirus S protein, SARS coronavirus Spike Protein, SARS CoV, SARS CoV S glycoprotein, SARS CoV Spike protein, SARSCoV, SARS-CoV S protein, SARS-COV Spike, Severe acute respiratory syndrome spike glycoprotein, Severe acute respiratory syndrome virus spike glycoprotein, Spike glycoprotein, VGL2
Immunogen: The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 755-769 (C-QYGSFCTQLNRALTG) from the S (Spike glycoprotein)(Spike protein 2) for the Human SARS coronavirus (Genbank accession no. YP_009724390)
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: Sars Spike Protein
Dilution: Western Blot 1:1000
Classification: Polyclonal
Form: Antisera
Regulatory Status: RUO
Formulation: Whole antisera with PBS and 0.2% Gelatin with 0.05% Sodium Azide
Gene ID (Entrez): 1489668
Isotype: IgG
Purification Method: Unpurified
Test Specificity: __
Concentration: __
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: SARS-CoV
Gene Alias: E2, E2 glycoprotein, Human coronavirus spike glycoprotein, Peplomer protein, S, S glycoprotein, SARS coronavirus S protein, SARS coronavirus Spike Protein, SARS CoV, SARS CoV S glycoprotein, SARS CoV Spike protein, SARSCoV, SARS-CoV S protein, SARS-COV Spike, Severe acute respiratory syndrome spike glycoprotein, Severe acute respiratory syndrome virus spike glycoprotein, Spike glycoprotein, VGL2
Immunogen: The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 553-570 (C-TESNKKFLPFQQFGRDIA from the S (Spike glycoprotein)(Spike protein 1) for the Human SARS coronavirus (Genbank accession no. YP_009724390)
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.