5092537
Smad3 Antibody, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 4 publications
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
Smad3
Dilution
Western Blot 0.5-2 ug/ml, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 5 ug/ml
Classification
Polyclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Porcine, Bovine, Canine, Primate
Gene Accession No.
NP_005893
Gene ID (Entrez)
4088
Immunogen
A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration
1.0 mg/mL
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Host Species
Rabbit
Research Discipline
Wnt Signaling Pathway
Formulation
PBS with 0.05% Sodium Azide
Gene Alias
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbols
SMAD3
Isotype
IgG
Purification Method
Protein G purified
Test Specificity
Cross reacts with Bovine, Dog, Human, Mouse, Pig, Rat and Rhesus Monkey. Predicted to react with Zebrafish, Xenopus and Chicken. The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, dog, pig, bovine and rhesus monkey, and 90% homologous in chicken, xenopus and zebrafish.
Description
- Smad3 Polyclonal specifically detects Smad3 in Human, Mouse, Rat, Porcine, Bovine, Canine, Primate samples
- It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
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Antigen: Smad3
Dilution: Western Blot 0.5-2 ug/ml, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 5 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Bovine, Canine, Primate
Gene Accession No.: NP_005893
Gene ID (Entrez): 4088
Immunogen: A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Wnt Signaling Pathway
Formulation: PBS with 0.05% Sodium Azide
Gene Alias: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbols: SMAD3
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: Cross reacts with Bovine, Dog, Human, Mouse, Pig, Rat and Rhesus Monkey. Predicted to react with Zebrafish, Xenopus and Chicken. The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, dog, pig, bovine and rhesus monkey, and 90% homologous in chicken, xenopus and zebrafish.
Antigen:
Smad3
Dilution:
Western Blot 0.5-2 ug/ml, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 5 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Porcine, Bovine, Canine, Primate
Gene Accession No.:
NP_005893
Gene ID (Entrez):
4088
Immunogen:
A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
1.0 mg/mL
Applications:
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Wnt Signaling Pathway
Formulation:
PBS with 0.05% Sodium Azide
Gene Alias:
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbols:
SMAD3
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
Cross reacts with Bovine, Dog, Human, Mouse, Pig, Rat and Rhesus Monkey. Predicted to react with Zebrafish, Xenopus and Chicken. The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, dog, pig, bovine and rhesus monkey, and 90% homologous in chicken, xenopus and zebrafish.
Antigen: Smad4
Dilution: Western Blot 1-3 μg/mL, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Monkey
Gene Accession No.: Q13485
Gene ID (Entrez): 4089
Immunogen: This antibody was developed against synthetic peptides corresponding to amino acids 186-223 and amino acids 509-544 of human SMAD4 (GenBank Accession No. NP_005350.1). These peptide sequences are specific to SMAD4, and have high homology to SMAD4 in multiple species.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Signal Transduction, Stem Cell Signaling Pathway
Formulation: 0.1 ml PBS with 0.05% Sodium Azide
Gene Alias: DPC4SMAD, mothers against DPP homolog 4 (Drosophila), hSMAD4, JIP, MAD homolog 4, MADH4mothers against decapentaplegic homolog 4, mothers against decapentaplegic homolog 4, mothers against decapentaplegic, Drosophila, homolog of, 4, SMAD 4, SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila), SMAD, mothers against DPP homolog 4, Smad4
Gene Symbols: SMAD4
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: __
Antigen:
Smad4
Dilution:
Western Blot 1-3 μg/mL, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Monkey
Gene Accession No.:
Q13485
Gene ID (Entrez):
4089
Immunogen:
This antibody was developed against synthetic peptides corresponding to amino acids 186-223 and amino acids 509-544 of human SMAD4 (GenBank Accession No. NP_005350.1). These peptide sequences are specific to SMAD4, and have high homology to SMAD4 in multiple species.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
1.0 mg/mL
Applications:
Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
Signal Transduction, Stem Cell Signaling Pathway
Formulation:
0.1 ml PBS with 0.05% Sodium Azide
Gene Alias:
DPC4SMAD, mothers against DPP homolog 4 (Drosophila), hSMAD4, JIP, MAD homolog 4, MADH4mothers against decapentaplegic homolog 4, mothers against decapentaplegic homolog 4, mothers against decapentaplegic, Drosophila, homolog of, 4, SMAD 4, SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila), SMAD, mothers against DPP homolog 4, Smad4
Gene Symbols:
SMAD4
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
__
Antigen: SMAD6
Dilution: Western Blot 0.5 - 2 ug/mL. Use reported by customer review, Simple Western 1:50, Immunohistochemistry 5 - 10 ug/mL, Immunocytochemistry/Immunofluorescence 1 - 5 ug/mL. Use reported in scientific literature (PMID 20032053), Immunohistochemistry-Paraffin 5 - 10 ug/mL. Use reported in scientific literature (PMID 20032053)
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Primate, Monkey, Sheep
Gene Accession No.: O43541
Gene ID (Entrez): 4091
Immunogen: This antibody was developed against 2 synthetic peptides found between amino acids 30-150 and 300-400 of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6 in multiple species.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: __
Formulation: PBS with 0.05% Sodium Azide
Gene Alias: hSMAD6, HsT17432, MAD homolog 6, MADH6SMAD, mothers against DPP homolog 6 (Drosophila), MADH7, mothers against decapentaplegic homolog 6, Mothers against decapentaplegic, drosophila, homolog of, 6, Mothers against DPP homolog 6, SMAD 6, SMAD family member 6MAD, mothers against decapentaplegic homolog 6 (Drosophila), SMAD, mothers against DPP homolog 6, Smad6
Gene Symbols: SMAD6
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: A 52 kDa band is observed.
Antigen:
SMAD6
Dilution:
Western Blot 0.5 - 2 ug/mL. Use reported by customer review, Simple Western 1:50, Immunohistochemistry 5 - 10 ug/mL, Immunocytochemistry/Immunofluorescence 1 - 5 ug/mL. Use reported in scientific literature (PMID 20032053), Immunohistochemistry-Paraffin 5 - 10 ug/mL. Use reported in scientific literature (PMID 20032053)
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Primate, Monkey, Sheep
Gene Accession No.:
O43541
Gene ID (Entrez):
4091
Immunogen:
This antibody was developed against 2 synthetic peptides found between amino acids 30-150 and 300-400 of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6 in multiple species.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
1.0 mg/mL
Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
__
Formulation:
PBS with 0.05% Sodium Azide
Gene Alias:
hSMAD6, HsT17432, MAD homolog 6, MADH6SMAD, mothers against DPP homolog 6 (Drosophila), MADH7, mothers against decapentaplegic homolog 6, Mothers against decapentaplegic, drosophila, homolog of, 6, Mothers against DPP homolog 6, SMAD 6, SMAD family member 6MAD, mothers against decapentaplegic homolog 6 (Drosophila), SMAD, mothers against DPP homolog 6, Smad6
Gene Symbols:
SMAD6
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
A 52 kDa band is observed.
Antigen: Smad7
Dilution: Western Blot 2-5 ug/ml, ELISA reported in scientific literature (PMID 32108968), Immunohistochemistry 5-10 ug/ml, Immunoprecipitation, Immunohistochemistry-Paraffin 5 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Sheep
Gene Accession No.: NP_005895
Gene ID (Entrez): 4092
Immunogen: This antibody was raised against two peptides made from within amino acids 10 and 50 of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: 1.0 mg/ml
Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: __
Formulation: PBS with 0.05% Sodium Azide
Gene Alias: CRCS3, FLJ16482, hSMAD7, MAD homolog 7, MAD homolog 8, MAD, mothers against decapentaplegic homolog 7 (Drosophila), MADH7mothers against decapentaplegic homolog 7, MADH8, Mothers against decapentaplegic homolog 8, Mothers against decapentaplegic, drosophila, homolog of, 7, Mothers against DPP homolog 7, Mothers against DPP homolog 8, SMAD 7, SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7, SMAD, mothers against DPP homolog 7, SMAD, mothers against DPP homolog 7 (Drosophila), Smad7
Gene Symbols: SMAD7
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: This antibody may cross-react with SMAD6 depending on cell or tissue-specific expression.
Antigen:
Smad7
Dilution:
Western Blot 2-5 ug/ml, ELISA reported in scientific literature (PMID 32108968), Immunohistochemistry 5-10 ug/ml, Immunoprecipitation, Immunohistochemistry-Paraffin 5 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Sheep
Gene Accession No.:
NP_005895
Gene ID (Entrez):
4092
Immunogen:
This antibody was raised against two peptides made from within amino acids 10 and 50 of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
1.0 mg/ml
Applications:
Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
__
Formulation:
PBS with 0.05% Sodium Azide
Gene Alias:
CRCS3, FLJ16482, hSMAD7, MAD homolog 7, MAD homolog 8, MAD, mothers against decapentaplegic homolog 7 (Drosophila), MADH7mothers against decapentaplegic homolog 7, MADH8, Mothers against decapentaplegic homolog 8, Mothers against decapentaplegic, drosophila, homolog of, 7, Mothers against DPP homolog 7, Mothers against DPP homolog 8, SMAD 7, SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7, SMAD, mothers against DPP homolog 7, SMAD, mothers against DPP homolog 7 (Drosophila), Smad7
Gene Symbols:
SMAD7
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
This antibody may cross-react with SMAD6 depending on cell or tissue-specific expression.