CD34 Monoclonal Antibody (RAM34), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin
• It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells
• CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells
• RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity
• CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion
• Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers
• Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations
• CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible
• CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors
• CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions
• CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells
• Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components
• CD34 is thought to have a role in presenting carbohydrate ligands to selectins
• The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction
• Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.