Axl Monoclonal Antibody (MAXL8DS), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: This MAXL8DS monoclonal antibody recognizes mouse Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3
• Within hematopoietic compartment the highest expression of Axl can be observed on dendritic cells and macrophages
• Stimulation with TLR ligands has been shown to additionally upregulate this expression
• This MAXL8DS antibody will work in flow cytometry on both native and fixed/permeabilized cells
• Applications Reported: This MAXL8DS antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MAXL8DS antibody has been tested by flow cytometric analysis of mouse bone marrow cells cultured with GM-CSF
• This may be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma
• The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation
• TAM receptors are also involved in phagocytosis of apoptotic cells
• Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies
• In addition, Axl may function as a putative entry receptor for filoviruses
• Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells
• Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C
• Soluble Axl is generated by proteolytic cleavage of the membrane form
• Increased plasma levels may be indicative of inflammation and cancer.