5317127
Phospho-Tyrosine Antibody, R&D Systems™
Mouse Monoclonal Antibody has been used in 1 publication
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
Phospho-Tyrosine
Dilution
Western Blot 1 ug/mL, Simple Western 50 ug/mL, Immunoprecipitation 5 ug/500 ug cell lysate, Immunocytochemistry 8-25 ug/mL
Classification
Monoclonal
Host Species
Mouse
Target Species
Multi-species
Gene Alias
2 amino 3(4 hydroxyphenyl) propanoic acid, 4 hydroxyphenylalanine, PhosphoTyrosine, pTyrosine, Tyrosine
Isotype
IgG1
Purification Method
Protein A or G purified from hybridoma culture supernatant
Content And Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone
179003
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry
Conjugate
Unconjugated
Regulatory Status
RUO
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen
KLH-coupled phospho-tyrosine synthetic peptide
Primary or Secondary
Primary
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity
Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequence. No cross-reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.
Related Products
Description
- Phospho-Tyrosine Monoclonal specifically detects Phospho-Tyrosine in Multi-Species samples
- It is validated for Western Blot, Simple Western, Immunoprecipitation, Immunocytochemistry.
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Antigen: Phospho-Tyrosine
Dilution: Western Blot 1 ug/mL, Simple Western 50 ug/mL, Immunoprecipitation 5 ug/500 ug cell lysate, Immunocytochemistry 8-25 ug/mL
Classification: Monoclonal
Host Species: Mouse
Target Species: Multi-species
Gene Alias: 2 amino 3(4 hydroxyphenyl) propanoic acid, 4 hydroxyphenylalanine, PhosphoTyrosine, pTyrosine, Tyrosine
Isotype: IgG1
Purification Method: Protein A or G purified from hybridoma culture supernatant
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone: 179003
Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen: KLH-coupled phospho-tyrosine synthetic peptide
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequence. No cross-reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.
Antigen:
Phospho-Tyrosine
Dilution:
Western Blot 1 ug/mL, Simple Western 50 ug/mL, Immunoprecipitation 5 ug/500 ug cell lysate, Immunocytochemistry 8-25 ug/mL
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Multi-species
Gene Alias:
2 amino 3(4 hydroxyphenyl) propanoic acid, 4 hydroxyphenylalanine, PhosphoTyrosine, pTyrosine, Tyrosine
Isotype:
IgG1
Purification Method:
Protein A or G purified from hybridoma culture supernatant
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone:
179003
Applications:
Western Blot, Immunoprecipitation, Immunocytochemistry
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen:
KLH-coupled phospho-tyrosine synthetic peptide
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequence. No cross-reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.
Antigen: EGF
Dilution: Western Blot 1 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
Classification: Monoclonal
Host Species: Rat
Target Species: Mouse
Gene Alias: beta-urogastrone, epidermal growth factor, epidermal growth factor (beta-urogastrone), hEGF, HOMG4, pro-epidermal growth factor, URG, Urogastrone
Isotype: IgG2a
Purification Method: Protein A or G purified from hybridoma culture supernatant
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone: 262930
Applications: Western Blot, ELISA
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen: E. coli-derived recombinant mouse EGF Asn977-Arg1029 Accession # P01132
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects mouse EGF in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) Amphiregulin, rmBetacellulin, rhEGF, rmEpigen, rmEpiregulin, rhHB-EGF, rhNRG-1 alpha , rhNRG1-beta 1, or rhTGF-alpha .
Antigen:
EGF
Dilution:
Western Blot 1 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
Classification:
Monoclonal
Host Species:
Rat
Target Species:
Mouse
Gene Alias:
beta-urogastrone, epidermal growth factor, epidermal growth factor (beta-urogastrone), hEGF, HOMG4, pro-epidermal growth factor, URG, Urogastrone
Isotype:
IgG2a
Purification Method:
Protein A or G purified from hybridoma culture supernatant
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone:
262930
Applications:
Western Blot, ELISA
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen:
E. coli-derived recombinant mouse EGF Asn977-Arg1029 Accession # P01132
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects mouse EGF in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) Amphiregulin, rmBetacellulin, rhEGF, rmEpigen, rmEpiregulin, rhHB-EGF, rhNRG-1 alpha , rhNRG1-beta 1, or rhTGF-alpha .
Antigen: EMMPRIN/CD147
Dilution: Western Blot 0.2 ug/mL, ELISA, Knockout Validated
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Gene Alias: 5F7, basigin, basigin (Ok blood group), BSG, CD147, CD147 antigen, Collagenase stimulatory factor, EMMPRINTCSF, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M6, OK, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor
Isotype: IgG2b
Purification Method: Protein A or G purified from hybridoma culture supernatant
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone: 109403
Applications: Western Blot, ELISA, Immunoassay
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen: Mouse myeloma cell line NS0-derived recombinant human EMMPRIN/CD147 Ala22-His205 Accession # Q54A51
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects human EMMPRIN/CD147 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse EMMPRIN is observed.
Antigen:
EMMPRIN/CD147
Dilution:
Western Blot 0.2 ug/mL, ELISA, Knockout Validated
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Gene Alias:
5F7, basigin, basigin (Ok blood group), BSG, CD147, CD147 antigen, Collagenase stimulatory factor, EMMPRINTCSF, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M6, OK, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor
Isotype:
IgG2b
Purification Method:
Protein A or G purified from hybridoma culture supernatant
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone:
109403
Applications:
Western Blot, ELISA, Immunoassay
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen:
Mouse myeloma cell line NS0-derived recombinant human EMMPRIN/CD147 Ala22-His205 Accession # Q54A51
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects human EMMPRIN/CD147 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse EMMPRIN is observed.
Antigen: Endoglin/CD105
Dilution: Flow Cytometry 10 uL/10^6 cells
Classification: Monoclonal
Host Species: Rat
Target Species: Mouse
Gene Alias: CD105, CD105 antigen, endoglin, ENDOsler-Rendu-Weber syndrome 1, ENG, HHT1FLJ41744, ORW, ORW1
Isotype: IgG2a
Purification Method: Protein A or G purified from hybridoma culture supernatant
Content And Storage: Protect from light.Do not freeze.12 months from date of receipt, 2 to 8 degreesC as supplied.
Clone: 209701
Applications: Flow Cytometry
Conjugate: Fluorescein
Regulatory Status: RUO
Formulation: Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide
Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105 Glu21-Gly581 (predicted) Accession # NP_031958
Primary or Secondary: Primary
Reconstitution: __
Test Specificity: Detects mouse Endoglin/CD105 in direct ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant human Endoglin/CD105.
Antigen:
Endoglin/CD105
Dilution:
Flow Cytometry 10 uL/10^6 cells
Classification:
Monoclonal
Host Species:
Rat
Target Species:
Mouse
Gene Alias:
CD105, CD105 antigen, endoglin, ENDOsler-Rendu-Weber syndrome 1, ENG, HHT1FLJ41744, ORW, ORW1
Isotype:
IgG2a
Purification Method:
Protein A or G purified from hybridoma culture supernatant
Content And Storage:
Protect from light.Do not freeze.12 months from date of receipt, 2 to 8 degreesC as supplied.
Clone:
209701
Applications:
Flow Cytometry
Conjugate:
Fluorescein
Regulatory Status:
RUO
Formulation:
Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide
Immunogen:
Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105 Glu21-Gly581 (predicted) Accession # NP_031958
Primary or Secondary:
Primary
Reconstitution:
__
Test Specificity:
Detects mouse Endoglin/CD105 in direct ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant human Endoglin/CD105.