Bcl-2 Antibody (100/D5), Novus Biologicals™
Mouse Monoclonal Antibody has been used in 7 publications
Manufacturer: Fischer Scientific
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Antigen
Bcl-2
Concentration
0.2mg/mL
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Formulation
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias
apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
Gene Symbols
BCL2
Isotype
IgG1 κ
Purification Method
Protein G purified
Content And Storage
Store at 4C.
Molecular Weight of Antigen
25 kDa
Clone
100/D5
Dilution
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Tumor Suppressors
Gene Accession No.
P10415
Gene ID (Entrez)
596
Immunogen
The synthetic peptide corresponding to amino acids range from 41-64 (GAAPAPGIFSSQPG-Cys), of human Bcl-2 was used as immunogen to generate the antibody; GenBank no. NP_000648.2 (Pezzella et al, 1990).
Primary or Secondary
Primary
Reconstitution
Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Test Specificity
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Description
- Bcl-2 Monoclonal specifically detects Bcl-2 in Human, Mouse (Negative), Rat (Negative) samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular).
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Antigen: Bcl-2
Concentration: 0.2mg/mL
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
Gene Symbols: BCL2
Isotype: IgG1 κ
Purification Method: Protein G purified
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 25 kDa
Clone: 100/D5
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Tumor Suppressors
Gene Accession No.: P10415
Gene ID (Entrez): 596
Immunogen: The synthetic peptide corresponding to amino acids range from 41-64 (GAAPAPGIFSSQPG-Cys), of human Bcl-2 was used as immunogen to generate the antibody; GenBank no. NP_000648.2 (Pezzella et al, 1990).
Primary or Secondary: Primary
Reconstitution: Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Test Specificity: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Antigen: __
Concentration: __
Classification: __
Form: __
Regulatory Status: __
Formulation: __
Gene Alias: __
Gene Symbols: __
Isotype: __
Purification Method: __
Content And Storage: __
Molecular Weight of Antigen: __
Clone: __
Dilution: __
Conjugate: __
Host Species: __
Research Discipline: __
Gene Accession No.: __
Gene ID (Entrez): __
Immunogen: __
Primary or Secondary: __
Reconstitution: __
Test Specificity: __
Antigen: CD63
Concentration: 1.0 mg/mL
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% Sodium Azide
Gene Alias: CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 molecule, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, melanoma 1 antigen, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1lysosome-associated membrane glycoprotein 3, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, tspan-30, TSPAN30granulophysin
Gene Symbols: CD63
Isotype: IgG2a λ
Purification Method: Protein G purified
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: __
Clone: 7G4.2E8
Dilution: Western Blot 2.0 ug/ml, Flow Cytometry 2 ug 1 million cells, Flow (Intracellular) 2 ug 1 million cells
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Autophagy, Cancer, Cardiovascular Biology, Cytokine Research, Immunology, Innate Immunity, Lysosome Markers, Metastasis
Gene Accession No.: P41731
Gene ID (Entrez): 967
Immunogen: Partial recombinant mouse CD63 protein (between amino acids 100-250) [UniProt P41731]
Primary or Secondary: Primary
Reconstitution: __
Test Specificity: __
