Cytokeratin 19 Antibody (A53-B/A2.26), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Cytokeratin 19
Concentration
0.2mg/mL
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Formulation
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias
CK19, CK-19, cytokeratin 19, Cytokeratin-19,40-kDa keratin intermediate filament, K19cytokeratin-19, K1CS, keratin 19, keratin, type I cytoskeletal 19, keratin, type I, 40-kd, keratin-19, MGC15366
Gene Symbols
KRT19
Isotype
IgG2a κ
Purification Method
Protein G purified
Test Specificity
This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Clone
A53-B/A2.26
Dilution
Western Blot 0.5-1ug/ml, Simple Western 10 ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Neuroscience, Stem Cell Markers
Gene Accession No.
P08727
Gene ID (Entrez)
3880
Immunogen
Human breast cancer MCF-7 cells were used as the immunogen for the Keratin-19 antibody.
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Description
- Cytokeratin 19 Monoclonal specifically detects Cytokeratin 19 in Human samples
- It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
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Antigen: Cytokeratin 19
Concentration: 0.2mg/mL
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: CK19, CK-19, cytokeratin 19, Cytokeratin-19,40-kDa keratin intermediate filament, K19cytokeratin-19, K1CS, keratin 19, keratin, type I cytoskeletal 19, keratin, type I, 40-kd, keratin-19, MGC15366
Gene Symbols: KRT19
Isotype: IgG2a κ
Purification Method: Protein G purified
Test Specificity: This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Clone: A53-B/A2.26
Dilution: Western Blot 0.5-1ug/ml, Simple Western 10 ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Neuroscience, Stem Cell Markers
Gene Accession No.: P08727
Gene ID (Entrez): 3880
Immunogen: Human breast cancer MCF-7 cells were used as the immunogen for the Keratin-19 antibody.
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Antigen: VCAM-1/CD106
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: __
Regulatory Status: RUO
Formulation: Aqueous, may contain carrier protein/stabilizer. with 0.09% Sodium Azide
Gene Alias: CD106, CD106 antigen, DKFZp779G2333, INCAM-100, L1CAM, MGC99561, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, V-CAM 1, VCAM1, VCAM-1
Gene Symbols: VCAM1
Isotype: IgG1 κ
Purification Method: Affinity Purified
Test Specificity: __
Clone: MR106
Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer, Mesenchymal Stem Cell Markers, Stem Cell Markers
Gene Accession No.: __
Gene ID (Entrez): 7412
Immunogen: The immunogen for this antibody was CD106.
Primary or Secondary: Primary
Content And Storage: Store at 4C. Do not freeze.
Antigen: CD98
Concentration: 1.0 mg/mL
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% Sodium Azide
Gene Alias: 4F2, 4F2hc, 4T2HC, antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC, CD98, CD98 antigen, CD98 heavy chain, CD98HC, heavy chain, Lymphocyte activation antigen 4F2 large subunit, MDU1antigen defined by monoclonal 4F2, heavy chain, monoclonal 44D7, NACAE4F2 cell-surface antigen heavy chain, solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen
Gene Symbols: SLC3A2
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: __
Clone: 1C11.7E3
Dilution: Western Blot 2 ug ml, Flow Cytometry 2.5 ug million cells, Immunohistochemistry 1:200 - 1:1000, Immunohistochemistry-Paraffin 1:200 - 1:1000, Flow (Cell Surface)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cell Biology, Cellular Markers, Immunology, Lipid and Metabolism, Plasma Membrane Markers, Signal Transduction
Gene Accession No.: P08195
Gene ID (Entrez): 6520
Immunogen: Partial recominant human CD98 (between amino acids 200-400) [UniProt P08195]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
