MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free, Novus Biologicals™
Rat Monoclonal Antibody has been used in 11 publications
Manufacturer: Fischer Scientific
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Antigen
MHC Class II
Concentration
0.5 mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Mouse
Gene Alias
class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
Gene Symbols
HLA-DMA
Isotype
IgG2b κ
Purification Method
Affinity Purified
Test Specificity
The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Clone
M5/114.15.2
Dilution
Western Blot 1:100-1:2000, Flow Cytometry < 0.125 ug/10^6 cells, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification
Monoclonal
Host Species
Rat
Research Discipline
Adaptive Immunity, Asthma, Cell Biology, Diabetes Research, Immunology, Major Histocompatibility Complexes
Formulation
Aqueous, may contain carrier protein/stabilizer. with 0.09% Sodium Azide
Gene ID (Entrez)
3108
Immunogen
The immunogen for this antibody was MHC Class II.
Primary or Secondary
Primary
Content And Storage
Store at 4C. Do not freeze.
Related Products
Description
- MHC class II (I-A/I-E) Monoclonal specifically detects MHC class II (I-A/I-E) in Human, Mouse samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
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Antigen: MHC Class II
Concentration: 0.5 mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
Gene Symbols: HLA-DMA
Isotype: IgG2b κ
Purification Method: Affinity Purified
Test Specificity: The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Clone: M5/114.15.2
Dilution: Western Blot 1:100-1:2000, Flow Cytometry < 0.125 ug/10^6 cells, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Classification: Monoclonal
Host Species: Rat
Research Discipline: Adaptive Immunity, Asthma, Cell Biology, Diabetes Research, Immunology, Major Histocompatibility Complexes
Formulation: Aqueous, may contain carrier protein/stabilizer. with 0.09% Sodium Azide
Gene ID (Entrez): 3108
Immunogen: The immunogen for this antibody was MHC Class II.
Primary or Secondary: Primary
Content And Storage: Store at 4C. Do not freeze.
Antigen: MHC Class II
Concentration: 0.2 mg/mL
Applications: Flow Cytometry
Conjugate: PE-Cyanine5
Regulatory Status: RUO
Target Species: Mouse
Gene Alias: class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
Gene Symbols: HLA-DMA
Isotype: IgG2b κ
Purification Method: Affinity Purified
Test Specificity: The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Clone: M5/114.15.2
Dilution: Flow Cytometry 1:10-1:1000
Classification: Monoclonal
Host Species: Rat
Research Discipline: Adaptive Immunity, Asthma, Cell Biology, Diabetes Research, Immunology, Major Histocompatibility Complexes
Formulation: Aqueous buffer, may contain carrier protein/stabilizer (proprietary) with 0.09% Sodium Azide
Gene ID (Entrez): 3108
Immunogen: The immunogen for this antibody was MHC Class II.
Primary or Secondary: Primary
Content And Storage: Store at 4C. Do not freeze.
Antigen: Mucin 5AC
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Chicken, Feline, Primate, Rabbit, Bovine (Negative)
Gene Alias: gastric mucin, leB, lewis B blood group antigen, major airway glycoprotein, MUC5, mucin 5, subtypes A and C, tracheobronchial/gastric, mucin 5AC, oligomeric mucus/gel-forming, mucin 5AC, oligomeric mucus/gel-forming pseudogene, mucin-5 subtype AC, tracheobronchial, mucin-5AC, TBM, tracheobronchial mucin
Gene Symbols: MUC5AC
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: __
Clone: 45M1
Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1.0ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5ug/ml, CyTOF-ready
Classification: Monoclonal
Host Species: Mouse
Research Discipline: Extracellular Matrix
Formulation: 10mM PBS - BSA free with No Preservative
Gene ID (Entrez): 4586
Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
