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5324792

TAG-72 Antibody (CC49), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Manufacturer: Fischer Scientific

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Antigen

TAG-72

Concentration

0.2mg/mL

Applications

Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Human, Rat, Bovine, Canine

Gene Alias

CA 72-4, Protein TAG-72, tag 72, tag-72 Protein TAG-72

Immunogen

Purified TAG-72 protein

Primary or Secondary

Primary

Reconstitution

Protein A purified

Test Specificity

Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.

Clone

CC49

Dilution

Western Blot 1:200-1:400, Flow Cytometry 0.5ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen 1:200-1:400

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene ID (Entrez)

182875

Isotype

IgG1 κ

Purification Method

Protein A purified

Content And Storage

Store at 4C.

Molecular Weight of Antigen

220 kDa

Description

  • TAG-72 Monoclonal specifically detects TAG-72 in Human, Rat, Bovine, Canine samples
  • It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

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Img

Fischer Scientific

5324792

Mouse Monoclonal Antibody has been ...

Antigen:
TAG-72
Concentration:
0.2mg/mL
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Rat, Bovine, Canine
Gene Alias:
CA 72-4, Protein TAG-72, tag 72, tag-72 Protein TAG-72
Immunogen:
Purified TAG-72 protein
Primary or Secondary:
Primary
Reconstitution:
Protein A purified
Test Specificity:
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Clone:
CC49
Dilution:
Western Blot 1:200-1:400, Flow Cytometry 0.5ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen 1:200-1:400
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene ID (Entrez):
182875
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
220 kDa

Img

Fischer Scientific

5324806

Mouse Monoclonal Antibody has been ...

Antigen:
T-bet/TBX21
Concentration:
0.5 mg/mL
Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Mouse, Rat
Gene Alias:
T-bet, TBETT-PET, TBLYMT-cell-specific T-box transcription factor T-bet, T-box 21, T-box expressed in T cells, T-box protein 21, T-box transcription factor TBX21, Transcription factor TBLYM
Immunogen:
The immunogen for this antibody was raised against TBX21.
Primary or Secondary:
Primary
Reconstitution:
__
Test Specificity:
__
Clone:
39D
Dilution:
Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100
Classification:
Monoclonal
Form:
__
Regulatory Status:
RUO
Formulation:
PBS, pH 7.2 with 0.09% Sodium Azide
Gene ID (Entrez):
30009
Isotype:
IgG1
Purification Method:
Affinity Purified
Content And Storage:
Store at 4C. Do not freeze.
Molecular Weight of Antigen:
__

Img

Fischer Scientific

5324806

Mouse Monoclonal Antibody has been ...

Antigen:
T-bet/TBX21
Concentration:
0.5 mg/mL
Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Mouse, Rat
Gene Alias:
T-bet, TBETT-PET, TBLYMT-cell-specific T-box transcription factor T-bet, T-box 21, T-box expressed in T cells, T-box protein 21, T-box transcription factor TBX21, Transcription factor TBLYM
Immunogen:
The immunogen for this antibody was raised against TBX21.
Primary or Secondary:
Primary
Reconstitution:
__
Test Specificity:
__
Clone:
39D
Dilution:
Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100
Classification:
Monoclonal
Form:
__
Regulatory Status:
RUO
Formulation:
PBS, pH 7.2 with 0.09% Sodium Azide
Gene ID (Entrez):
30009
Isotype:
IgG1
Purification Method:
Affinity Purified
Content And Storage:
Store at 4C. Do not freeze.
Molecular Weight of Antigen:
__

Img

Fischer Scientific

5324808

Mouse Monoclonal Antibody...

Antigen:
T-bet/TBX21
Concentration:
0.2 mg/mL
Applications:
Flow Cytometry
Conjugate:
PerCP-Cyanine5.5
Host Species:
Mouse
Target Species:
Human, Mouse, Rhesus Macaque
Gene Alias:
T-bet, TBETT-PET, TBLYMT-cell-specific T-box transcription factor T-bet, T-box 21, T-box expressed in T cells, T-box protein 21, T-box transcription factor TBX21, Transcription factor TBLYM
Immunogen:
The immunogen for this antibody was TBet.
Primary or Secondary:
Primary
Reconstitution:
__
Test Specificity:
__
Clone:
4B10
Dilution:
Flow Cytometry 1:10-1:1000
Classification:
Monoclonal
Form:
__
Regulatory Status:
RUO
Formulation:
Aqueous buffer, may contain carrier protein/stabilizer (proprietary) with 0.09% Sodium Azide
Gene ID (Entrez):
30009
Isotype:
IgG1 κ
Purification Method:
Affinity Purified
Content And Storage:
Store at 4C. Do not freeze.
Molecular Weight of Antigen:
__