5349254
MilliporeSigma™ anti-CD4 PE-Cy7, Clone: RM4-5,
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD4
Applications
Flow Cytometry
Conjugate
PE-Cyanine7
Host Species
Rat
Research Discipline
Inflammation, Immunology
Formulation
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols
Cd4
Isotype
IgG2a κ
Purification Method
Purified by size-exclusion chromatography
Clone
RM4-5
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse
Gene Accession No.
P06332
Immunogen
Proprietary immunogen
Primary or Secondary
Primary
Content And Storage
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Description
- Specifically detects CD4 Clone: RM4-5 in Mouse samples, and it is validated for Flow Cytometry The RM4-5 antibody reacts with mouse CD4, a 55kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells
- The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation
- CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression
- If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will 'compete' for binding, i.e
- RM4-5 antibody is able to block GK1.5 antibody binding to cells
- In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
Compare Similar Items
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Antigen: CD4
Applications: Flow Cytometry
Conjugate: PE-Cyanine7
Host Species: Rat
Research Discipline: Inflammation, Immunology
Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols: Cd4
Isotype: IgG2a κ
Purification Method: Purified by size-exclusion chromatography
Clone: RM4-5
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Accession No.: P06332
Immunogen: Proprietary immunogen
Primary or Secondary: Primary
Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Antigen:
CD4
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Rat
Research Discipline:
Inflammation, Immunology
Formulation:
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols:
Cd4
Isotype:
IgG2a κ
Purification Method:
Purified by size-exclusion chromatography
Clone:
RM4-5
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Accession No.:
P06332
Immunogen:
Proprietary immunogen
Primary or Secondary:
Primary
Content And Storage:
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Antigen: CD4
Applications: Flow Cytometry
Conjugate: Red Fluor 710
Host Species: Rat
Research Discipline: Inflammation, Immunology
Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols: Cd4
Isotype: __
Purification Method: Purified by size-exclusion chromatography
Clone: RM4-5
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Accession No.: P06332
Immunogen: Proprietary immunogen
Primary or Secondary: Primary
Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Antigen:
CD4
Applications:
Flow Cytometry
Conjugate:
Red Fluor 710
Host Species:
Rat
Research Discipline:
Inflammation, Immunology
Formulation:
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols:
Cd4
Isotype:
__
Purification Method:
Purified by size-exclusion chromatography
Clone:
RM4-5
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Accession No.:
P06332
Immunogen:
Proprietary immunogen
Primary or Secondary:
Primary
Content And Storage:
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Antigen: __
Applications: __
Conjugate: __
Host Species: __
Research Discipline: __
Formulation: __
Gene Symbols: __
Isotype: __
Purification Method: __
Clone: __
Classification: __
Form: __
Regulatory Status: __
Target Species: __
Gene Accession No.: __
Immunogen: __
Primary or Secondary: __
Content And Storage: __
Antigen:
__
Applications:
__
Conjugate:
__
Host Species:
__
Research Discipline:
__
Formulation:
__
Gene Symbols:
__
Isotype:
__
Purification Method:
__
Clone:
__
Classification:
__
Form:
__
Regulatory Status:
__
Target Species:
__
Gene Accession No.:
__
Immunogen:
__
Primary or Secondary:
__
Content And Storage:
__
Antigen: CD90.2
Applications: Flow Cytometry
Conjugate: Violet Fluor 450 (V450)
Host Species: Rat
Research Discipline: Inflammation, Immunology
Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols: Thy1, Thy-1
Isotype: IgG2b
Purification Method: Purified by size-exclusion chromatography
Clone: 30-H12
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Accession No.: P01831
Immunogen: Proprietary immunogen
Primary or Secondary: Primary
Content And Storage: 2°C to 8°C for one year from date of shipment protected from light; Do not freeze
Antigen:
CD90.2
Applications:
Flow Cytometry
Conjugate:
Violet Fluor 450 (V450)
Host Species:
Rat
Research Discipline:
Inflammation, Immunology
Formulation:
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols:
Thy1, Thy-1
Isotype:
IgG2b
Purification Method:
Purified by size-exclusion chromatography
Clone:
30-H12
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Accession No.:
P01831
Immunogen:
Proprietary immunogen
Primary or Secondary:
Primary
Content And Storage:
2°C to 8°C for one year from date of shipment protected from light; Do not freeze