5379863
Human CD25/IL-2R alpha Antibody, R&D Systems™
Mouse Monoclonal Antibody has been used in 3 publications
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD25/IL-2R alpha
Dilution
Western Blot 1 ug/mL, Immunohistochemistry 8-25 ug/mL, Immunocytochemistry 8-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
Classification
Monoclonal
Host Species
Mouse
Target Species
Human
Gene Accession No.
P01589
Gene ID (Entrez)
3559
Immunogen
S. frugiperdainsect ovarian cell lineSf21-derived recombinant human CD25/IL-2 R alpha Glu22-Cys213 Accession # P01589
Primary or Secondary
Primary
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity
Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In Western blots, detects recombinant rat CD25/IL-2 R alpha but no cross-reactivity with recombinant mouse CD25/IL-2 R alpha . In sandwich immunoassays, this antibody does not cross-react or interfere with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-2 R beta , rhIL-3 R alpha , rhIL-4 R, rhIL-5 R alpha , rhIL-5 R beta , rhIL-6 R, or rhgp130.
Clone
24204
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA
Conjugate
Unconjugated
Regulatory Status
RUO
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias
CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL2R, IL2R alpha, IL-2R subunit alpha, IL-2Ra, IL2RA, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
Gene Symbols
IL2RA
Isotype
IgG1
Purification Method
Protein A or G purified from ascites
Content And Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Description
- CD25/IL-2R alpha Monoclonal specifically detects CD25/IL-2R alpha in Human samples
- It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA Capture (Matched Antibody Pair).
Compare Similar Items
Show Difference
Antigen: CD25/IL-2R alpha
Dilution: Western Blot 1 ug/mL, Immunohistochemistry 8-25 ug/mL, Immunocytochemistry 8-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Gene Accession No.: P01589
Gene ID (Entrez): 3559
Immunogen: S. frugiperdainsect ovarian cell lineSf21-derived recombinant human CD25/IL-2 R alpha Glu22-Cys213 Accession # P01589
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In Western blots, detects recombinant rat CD25/IL-2 R alpha but no cross-reactivity with recombinant mouse CD25/IL-2 R alpha . In sandwich immunoassays, this antibody does not cross-react or interfere with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-2 R beta , rhIL-3 R alpha , rhIL-4 R, rhIL-5 R alpha , rhIL-5 R beta , rhIL-6 R, or rhgp130.
Clone: 24204
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias: CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL2R, IL2R alpha, IL-2R subunit alpha, IL-2Ra, IL2RA, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
Gene Symbols: IL2RA
Isotype: IgG1
Purification Method: Protein A or G purified from ascites
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Antigen:
CD25/IL-2R alpha
Dilution:
Western Blot 1 ug/mL, Immunohistochemistry 8-25 ug/mL, Immunocytochemistry 8-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P01589
Gene ID (Entrez):
3559
Immunogen:
S. frugiperdainsect ovarian cell lineSf21-derived recombinant human CD25/IL-2 R alpha Glu22-Cys213 Accession # P01589
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In Western blots, detects recombinant rat CD25/IL-2 R alpha but no cross-reactivity with recombinant mouse CD25/IL-2 R alpha . In sandwich immunoassays, this antibody does not cross-react or interfere with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-2 R beta , rhIL-3 R alpha , rhIL-4 R, rhIL-5 R alpha , rhIL-5 R beta , rhIL-6 R, or rhgp130.
Clone:
24204
Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias:
CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL2R, IL2R alpha, IL-2R subunit alpha, IL-2Ra, IL2RA, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
Gene Symbols:
IL2RA
Isotype:
IgG1
Purification Method:
Protein A or G purified from ascites
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Antigen: CD28
Dilution: Western Blot 1 ug/mL, Agonist Activity 0.2-0.6 ug/mL, Immunocytochemistry 8-25 ug/mL
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Gene Accession No.: P10747
Gene ID (Entrez): 940
Immunogen: S. frugiperdainsect ovarian cell lineSf21-derived recombinant human CD28 Asn19-Pro152 Accession # P10747
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects human CD28 in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) CD28, rhCTLA4, or rmCTLA4.
Clone: 37407
Applications: Western Blot, In vitro Assay, Immunocytochemistry
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias: CD28, CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44
Gene Symbols: CD28
Isotype: IgG1
Purification Method: Protein A or G purified from ascites
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Antigen:
CD28
Dilution:
Western Blot 1 ug/mL, Agonist Activity 0.2-0.6 ug/mL, Immunocytochemistry 8-25 ug/mL
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P10747
Gene ID (Entrez):
940
Immunogen:
S. frugiperdainsect ovarian cell lineSf21-derived recombinant human CD28 Asn19-Pro152 Accession # P10747
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects human CD28 in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) CD28, rhCTLA4, or rmCTLA4.
Clone:
37407
Applications:
Western Blot, In vitro Assay, Immunocytochemistry
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias:
CD28, CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44
Gene Symbols:
CD28
Isotype:
IgG1
Purification Method:
Protein A or G purified from ascites
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Antigen: CD28
Dilution: Western Blot 1 ug/mL, Agonist Activity 0.2-0.6 ug/mL, Immunocytochemistry 8-25 ug/mL
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Gene Accession No.: P10747
Gene ID (Entrez): 940
Immunogen: S. frugiperdainsect ovarian cell lineSf21-derived recombinant human CD28 Asn19-Pro152 Accession # P10747
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects human CD28 in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) CD28, rhCTLA4, or rmCTLA4.
Clone: 37407
Applications: Western Blot, In vitro Assay, Immunocytochemistry
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias: CD28, CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44
Gene Symbols: CD28
Isotype: IgG1
Purification Method: Protein A or G purified from ascites
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Antigen:
CD28
Dilution:
Western Blot 1 ug/mL, Agonist Activity 0.2-0.6 ug/mL, Immunocytochemistry 8-25 ug/mL
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P10747
Gene ID (Entrez):
940
Immunogen:
S. frugiperdainsect ovarian cell lineSf21-derived recombinant human CD28 Asn19-Pro152 Accession # P10747
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects human CD28 in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) CD28, rhCTLA4, or rmCTLA4.
Clone:
37407
Applications:
Western Blot, In vitro Assay, Immunocytochemistry
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias:
CD28, CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44
Gene Symbols:
CD28
Isotype:
IgG1
Purification Method:
Protein A or G purified from ascites
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Antigen: CD40 Ligand/TNFSF5
Dilution: Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 8-25 ug/mL, Blockade of Receptor-ligand Interaction, CyTOF-ready
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Gene Accession No.: P29965
Gene ID (Entrez): 959
Immunogen: E. coli-derived recombinant human CD40 Ligand/TNFSF5 Leu50-Leu261 Accession # P29965
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects human CD40 Ligand/TNFSF5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CD27 Ligand, rhCD30 Ligand, or recombinant mouse CD40 Ligand is observed.
Clone: 40804
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Blockade of Receptor-ligand Interaction, CyTOF
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias: CD154, CD154 antigen, CD40 antigen ligand, CD40 ligand, CD40L, CD40-L, CD40LG, CD40LIGM, gp39, hCD40L, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5
Gene Symbols: CD40LG
Isotype: IgG2b
Purification Method: Protein A or G purified from hybridoma culture supernatant
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Antigen:
CD40 Ligand/TNFSF5
Dilution:
Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 8-25 ug/mL, Blockade of Receptor-ligand Interaction, CyTOF-ready
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P29965
Gene ID (Entrez):
959
Immunogen:
E. coli-derived recombinant human CD40 Ligand/TNFSF5 Leu50-Leu261 Accession # P29965
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects human CD40 Ligand/TNFSF5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CD27 Ligand, rhCD30 Ligand, or recombinant mouse CD40 Ligand is observed.
Clone:
40804
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Blockade of Receptor-ligand Interaction, CyTOF
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias:
CD154, CD154 antigen, CD40 antigen ligand, CD40 ligand, CD40L, CD40-L, CD40LG, CD40LIGM, gp39, hCD40L, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5
Gene Symbols:
CD40LG
Isotype:
IgG2b
Purification Method:
Protein A or G purified from hybridoma culture supernatant
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.