5379979

Mouse IL-10 Antibody, R&D Systems™

Rat Monoclonal Antibody has been used in 36 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

IL-10

Dilution

Western Blot 1 ug/mL, Neutralization 0.005-0.015 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification

Monoclonal

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P18893

Gene ID (Entrez)

3586

Immunogen

E. coli-derived recombinant mouse IL-10 Accession # P18893

Primary or Secondary

Primary

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity

Detects mouse IL-10 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 8% cross-reactivity with recombinant rat IL-10 and 1% cross-reactivity with recombinant cotton rat IL-10 is observed. In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 R, rhIL-10 R, recombinant canine IL-10, recombinant feline IL-10, or recombinant porcine IL-10 is observed.

Clone

JES052A5

Applications

Western Blot, Neutralization, ELISA

Conjugate

Unconjugated

Regulatory Status

RUO

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. with No Preservative

Gene Alias

CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF

Gene Symbols

IL10

Isotype

IgG1

Purification Method

Protein A or G purified from hybridoma culture supernatant

Content And Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Description

  • IL-10 Monoclonal specifically detects IL-10 in Mouse samples
  • It is validated for Western Blot, Neutralization, ELISA Capture (Matched Antibody Pair).

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Fischer Scientific

5379979

Rat Monoclonal Antibody has been us...


Antigen:
IL-10

Dilution:
Western Blot 1 ug/mL, Neutralization 0.005-0.015 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification:
Monoclonal

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P18893

Gene ID (Entrez):
3586

Immunogen:
E. coli-derived recombinant mouse IL-10 Accession # P18893

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects mouse IL-10 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 8% cross-reactivity with recombinant rat IL-10 and 1% cross-reactivity with recombinant cotton rat IL-10 is observed. In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 R, rhIL-10 R, recombinant canine IL-10, recombinant feline IL-10, or recombinant porcine IL-10 is observed.

Clone:
JES052A5

Applications:
Western Blot, Neutralization, ELISA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. with No Preservative

Gene Alias:
CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF

Gene Symbols:
IL10

Isotype:
IgG1

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

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Fischer Scientific

5379980

Rat Monoclonal Antibody has been us...


Antigen:
IL-12 p70/IL-12A

Dilution:
Western Blot 1 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification:
Monoclonal

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
__

Gene ID (Entrez):
3592

Immunogen:
S. frugiperdainsect ovarian cell lineSf21-derived recombinant mouse IL-12

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects mouse IL-12 in ELISAs and Western blots. Detects an epitope in the p35 subunit. In sandwich ELISAs, does not cross-react with recombinant mouse IL-12 p40.

Clone:
48110

Applications:
Western Blot, ELISA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12, IL12 p70, IL-12 subunit p35, IL-12, subunit p35, IL12A, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35

Gene Symbols:
IL12A

Isotype:
IgG1

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Img

Fischer Scientific

5379982

Rat Monoclonal Antibody has been us...


Antigen:
IL-12/IL-23 p40

Dilution:
ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification:
Monoclonal

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
__

Gene ID (Entrez):
16160

Immunogen:
S. frugiperdainsect ovarian cell lineSf21-derived recombinant mouse IL-12 p40

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects mouse IL-12 p40 in ELISAs. Polymorphisms exist in the mouse IL-12 p40 sequence. This antibody recognizes an allelic epitope found in the p40 subunit of several mouse strains including BALB/c, C57BL/6, CBA, C3H, DBA/1, NOR, I29/J, and A.SW. This antibody does not recognize the allelic variant that occurs in strains AKR, DBA/2, NOD, NZB, NZC, NZO, NZW, or SJL (1). In ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse (rm) IL-23, 30% cross-reactivity with rmIL-12 p70, and no cross-reactivity with recombinant human (rh) IL-12 p70 or rhIL-12 p40.

Clone:
30517

Applications:
ELISA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
CLMF, CLMF p40, CLMF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL12 p40, IL-12 subunit p40, IL12B, IL-12B, IL-12BNK cell stimulatory factor chain 2, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40), interleukin-12 beta chain, interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NKSF, NKSF2, NKSF2IL12, subunit p40

Gene Symbols:
Il12b

Isotype:
IgG2b

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Img

Fischer Scientific

5379982

Rat Monoclonal Antibody has been us...


Antigen:
IL-12/IL-23 p40

Dilution:
ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification:
Monoclonal

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
__

Gene ID (Entrez):
16160

Immunogen:
S. frugiperdainsect ovarian cell lineSf21-derived recombinant mouse IL-12 p40

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects mouse IL-12 p40 in ELISAs. Polymorphisms exist in the mouse IL-12 p40 sequence. This antibody recognizes an allelic epitope found in the p40 subunit of several mouse strains including BALB/c, C57BL/6, CBA, C3H, DBA/1, NOR, I29/J, and A.SW. This antibody does not recognize the allelic variant that occurs in strains AKR, DBA/2, NOD, NZB, NZC, NZO, NZW, or SJL (1). In ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse (rm) IL-23, 30% cross-reactivity with rmIL-12 p70, and no cross-reactivity with recombinant human (rh) IL-12 p70 or rhIL-12 p40.

Clone:
30517

Applications:
ELISA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
CLMF, CLMF p40, CLMF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL12 p40, IL-12 subunit p40, IL12B, IL-12B, IL-12BNK cell stimulatory factor chain 2, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40), interleukin-12 beta chain, interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NKSF, NKSF2, NKSF2IL12, subunit p40

Gene Symbols:
Il12b

Isotype:
IgG2b

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.