5380075

Mouse Sonic Hedgehog/Shh N-Terminus Antibody, R&D Systems™

Rat Monoclonal Antibody has been used in 2 publications

Manufacturer: Fischer Scientific

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Antigen

Sonic Hedgehog/Shh

Dilution

Western Blot 2 ug/mL, Immunohistochemistry 8-25 ug/mL, Neutralization 5-15 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification

Monoclonal

Host Species

Rat

Target Species

Mouse

Gene Accession No.

Q62226

Gene ID (Entrez)

6469

Immunogen

E. coli-derived recombinant mouse Sonic Hedgehog Cys25-Gly198 (Lys122Arg) Accession # Q62226

Primary or Secondary

Primary

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity

Detects mouse Sonic Hedgehog in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Dhh (N-terminus; aa 23-198) is observed and no cross-reactivity with rmIhh (N-terminus; aa 66-240), rmDhh (C-terminus; aa 199-396), or rmShh (C-terminus; aa 199-437) is observed.

Clone

171018

Applications

Western Blot, Immunohistochemistry, Neutralization, ELISA

Conjugate

Unconjugated

Regulatory Status

RUO

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias

HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS

Gene Symbols

SHH

Isotype

IgG2a

Purification Method

Protein A or G purified from hybridoma culture supernatant

Content And Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Description

  • Sonic Hedgehog/Shh Monoclonal specifically detects Sonic Hedgehog/Shh in Mouse samples
  • It is validated for Western Blot, Immunohistochemistry, Neutralization, ELISA Capture (Matched Antibody Pair).

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Fischer Scientific

5380075

Rat Monoclonal Antibody has been us...


Antigen:
Sonic Hedgehog/Shh

Dilution:
Western Blot 2 ug/mL, Immunohistochemistry 8-25 ug/mL, Neutralization 5-15 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification:
Monoclonal

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
Q62226

Gene ID (Entrez):
6469

Immunogen:
E. coli-derived recombinant mouse Sonic Hedgehog Cys25-Gly198 (Lys122Arg) Accession # Q62226

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects mouse Sonic Hedgehog in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Dhh (N-terminus; aa 23-198) is observed and no cross-reactivity with rmIhh (N-terminus; aa 66-240), rmDhh (C-terminus; aa 199-396), or rmShh (C-terminus; aa 199-437) is observed.

Clone:
171018

Applications:
Western Blot, Immunohistochemistry, Neutralization, ELISA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS

Gene Symbols:
SHH

Isotype:
IgG2a

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Img

Fischer Scientific

5380078

Rat Monoclonal Antibody has been us...


Antigen:
ST2/IL-33R

Dilution:
Flow Cytometry 0.25 ug/10^6 cells, Neutralization 0.5-3 ug/mL, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification:
Monoclonal

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P14719

Gene ID (Entrez):
9173

Immunogen:
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R Ser27-Arg332 Accession # P14719

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects mouse ST2/IL-33 R in ELISAs.

Clone:
245707

Applications:
Flow Cytometry, Neutralization, CyTOF, ELISA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
DER4, DER4ST2growth stimulation-expressed, FIT-1, IL-1 R4, IL1R4, IL-1R4, IL-1RL1, IL-33 R, IL33R, IL-33R, interleukin 1 receptor-like 1, interleukin 1 receptor-related protein, interleukin-1 receptor-like 1, Ly84, MGC32623, Protein ST2, ST2L, ST2V, T1, T1homolog of mouse growth stimulation-expressed

Gene Symbols:
Il1rl1

Isotype:
IgG2b

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Img

Fischer Scientific

5380079

Mouse Monoclonal Antibody has been ...


Antigen:
Activin B/Inhibin beta B

Dilution:
Western Blot 1 ug/mL, Neutralization 0.6-3.0 ug/mL

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
Q53T31

Gene ID (Entrez):
3625

Immunogen:
Chinese hamster ovary cell line CHO-derived recombinant human Activin B Gly293-Ala407 Accession # Q53T31

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects human Activin B beta B subunit in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Inhibin B or rhActivin A is observed. In Western blots, approximately 20% cross-reactivity with rhInhibin B is observed and no cross-reactivity with rhActivin A or recombinant mouse Activin A is observed.

Clone:
146807

Applications:
Western Blot, Neutralization

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
activin AB beta polypeptide, Activin beta-B chain, INHBB, inhibin beta B chain, inhibin, beta B, inhibin, beta B (activin AB beta polypeptide), Inhibin, beta-2

Gene Symbols:
INHBB

Isotype:
IgG1

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Img

Fischer Scientific

5380086

Rat Monoclonal Antibody has been us...


Antigen:
Adiponectin/Acrp30

Dilution:
Western Blot 2 ug/mL

Classification:
Monoclonal

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
Q60994

Gene ID (Entrez):
9370

Immunogen:
Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin Glu18-Asn247 Accession # Q60994

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects full-length mouse Adiponectin (aa 18-247) in direct ELISAs and Western blots. Shows no cross-reactivity with recombinant human Adiponectin. Does not detect the C-terminal globular domain of mouse Adiponectin (aa 111-247).

Clone:
173205

Applications:
Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
ACDC, Acrp30, ACRP30ADPN, adipocyte, C1Q and collagen domain containing, adiponectin, adiponectin, C1Q and collagen domain containing, AdipoQ, ADIPQTL1, apM-1, ApM1, APM1APM-1, C1q and collagen domain-containing protein, GBP28, GBP28apM1, Gelatin-binding protein

Gene Symbols:
ADIPOQ

Isotype:
IgG2a

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.