5380113
His Tag Antibody, R&D Systems™
Mouse Monoclonal Antibody has been used in 44 publications
Manufacturer: Fischer Scientific
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Antigen
His Tag
Dilution
Western Blot 0.2 ug/mL, Simple Western 10 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunoaffinity Purification, CyTOF-ready
Classification
Monoclonal
Host Species
Mouse
Target Species
Multi-species
Gene Alias
6 His epitope tag, 6X His, 6X-His, H, HHHHHH epitope tag, HHHHHH tag, HIS, His tag, polyHistidine, Poly-histidine
Isotype
IgG1
Purification Method
Protein A or G purified from hybridoma culture supernatant
Content And Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone
AD1.1.10
Applications
Western Blot, Flow Cytometry, Affinity Purification, CyTOF
Conjugate
Unconjugated
Regulatory Status
RUO
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen
His-tagged peptide
Primary or Secondary
Primary
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.
Related Products
Description
- His Tag Monoclonal specifically detects His Tag in Multi-Species samples
- It is validated for Western Blot, Simple Western, Flow Cytometry, Immunoaffinity Purification, CyTOF-ready.
Compare Similar Items
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Antigen: His Tag
Dilution: Western Blot 0.2 ug/mL, Simple Western 10 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunoaffinity Purification, CyTOF-ready
Classification: Monoclonal
Host Species: Mouse
Target Species: Multi-species
Gene Alias: 6 His epitope tag, 6X His, 6X-His, H, HHHHHH epitope tag, HHHHHH tag, HIS, His tag, polyHistidine, Poly-histidine
Isotype: IgG1
Purification Method: Protein A or G purified from hybridoma culture supernatant
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone: AD1.1.10
Applications: Western Blot, Flow Cytometry, Affinity Purification, CyTOF
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen: His-tagged peptide
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.
Antigen:
His Tag
Dilution:
Western Blot 0.2 ug/mL, Simple Western 10 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunoaffinity Purification, CyTOF-ready
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Multi-species
Gene Alias:
6 His epitope tag, 6X His, 6X-His, H, HHHHHH epitope tag, HHHHHH tag, HIS, His tag, polyHistidine, Poly-histidine
Isotype:
IgG1
Purification Method:
Protein A or G purified from hybridoma culture supernatant
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone:
AD1.1.10
Applications:
Western Blot, Flow Cytometry, Affinity Purification, CyTOF
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen:
His-tagged peptide
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.
Antigen: M-CSFR/CD115
Dilution: Western Blot 2 ug/mL, Neutralization 4-16 ng/mL
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Gene Alias: CD115, CD115 antigen, C-FMS, colony stimulating factor 1 receptor, CSF1R, CSF-1-R, CSFR, EC 2.7.10.1, FMS proto-oncogene, FMSFIM2, macrophage colony stimulating factor I receptor, macrophage colony-stimulating factor 1 receptor, McDonough feline sarcoma viral (v-fms) oncogene homolog, MCSFR, M-CSFR, Proto-oncogene c-Fms
Isotype: IgG1
Purification Method: Protein A or G purified from hybridoma culture supernatant
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone: 61701
Applications: Western Blot, Neutralization
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen: Mouse myeloma cell line NS0-derived recombinant human M-CSF R/CD115 Ile20-Glu512 (Pro54Ala) Accession # P07333.2
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects human M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM-CSF R beta is observed.
Antigen:
M-CSFR/CD115
Dilution:
Western Blot 2 ug/mL, Neutralization 4-16 ng/mL
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Gene Alias:
CD115, CD115 antigen, C-FMS, colony stimulating factor 1 receptor, CSF1R, CSF-1-R, CSFR, EC 2.7.10.1, FMS proto-oncogene, FMSFIM2, macrophage colony stimulating factor I receptor, macrophage colony-stimulating factor 1 receptor, McDonough feline sarcoma viral (v-fms) oncogene homolog, MCSFR, M-CSFR, Proto-oncogene c-Fms
Isotype:
IgG1
Purification Method:
Protein A or G purified from hybridoma culture supernatant
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone:
61701
Applications:
Western Blot, Neutralization
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen:
Mouse myeloma cell line NS0-derived recombinant human M-CSF R/CD115 Ile20-Glu512 (Pro54Ala) Accession # P07333.2
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects human M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM-CSF R beta is observed.
Antigen: M-CSF
Dilution: Western Blot 1 ug/mL, Neutralization 0.005-0.020 ug/mL
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Gene Alias: colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, macrophage colony stimulating factor, macrophage colony-stimulating factor 1, MCSF, M-CSF, MCSFlanimostim, MGC31930
Isotype: IgG2a
Purification Method: Protein A or G purified from ascites
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone: 26730
Applications: Western Blot, Neutralization
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen: E. coli-derived recombinant human M-CSF
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects human M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse M-CSF.
Antigen:
M-CSF
Dilution:
Western Blot 1 ug/mL, Neutralization 0.005-0.020 ug/mL
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Gene Alias:
colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, macrophage colony stimulating factor, macrophage colony-stimulating factor 1, MCSF, M-CSF, MCSFlanimostim, MGC31930
Isotype:
IgG2a
Purification Method:
Protein A or G purified from ascites
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone:
26730
Applications:
Western Blot, Neutralization
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Immunogen:
E. coli-derived recombinant human M-CSF
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects human M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse M-CSF.
Antigen: MICA
Dilution: Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Blockade of Receptor-ligand Interaction, CyTOF-ready, Knockout Validated 0.25 ug/10^6 cells
Classification: Monoclonal
Host Species: Mouse
Target Species: Human
Gene Alias: FLJ60820, MGC111087, PERB11.1
Isotype: IgG2b
Purification Method: Protein A or G purified from hybridoma culture supernatant
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone: 159227
Applications: Western Blot, Flow Cytometry, Blockade of Receptor-ligand Interaction, CyTOF, Immunoassay
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. with No Preservative
Immunogen: BaF3 mouse pro-B cell line transfected with human MICA
Primary or Secondary: Primary
Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity: Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MICB is observed.
Antigen:
MICA
Dilution:
Western Blot 1 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Blockade of Receptor-ligand Interaction, CyTOF-ready, Knockout Validated 0.25 ug/10^6 cells
Classification:
Monoclonal
Host Species:
Mouse
Target Species:
Human
Gene Alias:
FLJ60820, MGC111087, PERB11.1
Isotype:
IgG2b
Purification Method:
Protein A or G purified from hybridoma culture supernatant
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Clone:
159227
Applications:
Western Blot, Flow Cytometry, Blockade of Receptor-ligand Interaction, CyTOF, Immunoassay
Conjugate:
Unconjugated
Regulatory Status:
RUO
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. with No Preservative
Immunogen:
BaF3 mouse pro-B cell line transfected with human MICA
Primary or Secondary:
Primary
Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity:
Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MICB is observed.