5539408

Cytokeratin 7 Antibody (KRT7/760), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Cytokeratin 7

Concentration

0.2mg/mL

Applications

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cytoskeleton Markers

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias

CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Gene Symbols

KRT7

Isotype

IgG1

Purification Method

Protein A or G purified

Test Specificity

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Clone

KRT7/760

Dilution

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Accession No.

P08729

Gene ID (Entrez)

3855

Immunogen

Recombinant full-length human KRT7 protein

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

55 kDa

Description

  • Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence Cytokeratin 7 Monoclonal specifically detects Cytokeratin 7 in Human samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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Fischer Scientific

5539408

Mouse Monoclonal Antibody...


Antigen:
Cytokeratin 7

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cytoskeleton Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Gene Symbols:
KRT7

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Clone:
KRT7/760

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P08729

Gene ID (Entrez):
3855

Immunogen:
Recombinant full-length human KRT7 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
55 kDa

Img

Fischer Scientific

5539410

Mouse Monoclonal Antibody has been ...


Antigen:
Cytokeratin 7

Concentration:
0.2mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cytoskeleton Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Gene Symbols:
KRT7

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Clone:
OV-TL12/30

Dilution:
Simple Western 1.0 ug/ml, Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100-1:200, Immunofluorescence 1:50 - 1:100

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P08729

Gene ID (Entrez):
3855

Immunogen:
OTN 11, ovarian carcinoma cell line

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
55 kDa

Img

Fischer Scientific

5539411

Mouse Monoclonal Antibody...


Antigen:
Cytokeratin 7

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cytoskeleton Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
CK7, CK-7, cytokeratin 7, Cytokeratin-7, K2C7, K7keratin, 55K type II cytoskeletal, keratin 7, keratin, type II cytoskeletal 7, keratin-7, MGC129731, MGC3625, Sarcolectin, SCLkeratin, simple epithelial type I, K7, type II mesothelial keratin K7, Type-II keratin Kb7

Gene Symbols:
KRT7

Isotype:
IgG1

Purification Method:
Protein A or G purified

Test Specificity:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Clone:
SPM270

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
__

Gene ID (Entrez):
3855

Immunogen:
OTN 11, ovarian carcinoma cell line

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
55 kDa

Img

Fischer Scientific

5539412

Mouse Monoclonal Antibody...


Antigen:
Cytokeratin 8

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cytoskeleton Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8

Gene Symbols:
KRT8

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma

Clone:
B22.1

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Porcine, Primate, Rabbit, Sheep, Chicken (Negative), Mouse (Negative), Rat (Negative), Xenopus (Negative)

Gene Accession No.:
__

Gene ID (Entrez):
3856

Immunogen:
Cytoskeletal preparation from HeLa cells

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
52.5 kDa