Dilution:
Western Blot, Flow Cytometry, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 10-20 ug/ml, Immunoprecipitation 2-10 ug, Immunohistochemistry-Paraffin 1:100-1:500, Immunohistochemistry-Frozen reported in scientific literature (PMID 32278100), Flow (Intracellular) 1 - 2.5 ug/mL, Knockout Validated
Classification:
Monoclonal
Research Discipline:
Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Hypoxia, Innate Immunity, Lipid and Metabolism, Macroautophagy, Mitophagy, Neuroscience
Formulation:
PBS with 0.02% Sodium Azide
Gene Alias:
ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B monoclonal, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B
Purification Method:
Protein G purified
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoassay
Target Species:
Human, Mouse, Rat
Gene Accession No.:
Q9GZQ8
Immunogen:
Recombinant monoclonal LC3B Antibody (1251A) was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8].
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.