CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin
• CD103 non-covalently associates with integrin beta7 and is expressed on all intraepithelial lymphocytes, a small subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC)
• Epithelial cell antigen E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium
• Applications Reported: This 2E7 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 2E7 antibody has been tested by flow cytometric analysis of mouse lymph node cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm)
• Most instruments will require a 685 LP mirror and 710/20 filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells
• In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain
• CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs
• CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes
• CD103 is part of the integrin family which are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain
• ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains
• In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen
• CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation
• Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.