CD335 (NKp46) Monoclonal Antibody (29A1.4), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335
• CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail
• Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-)
• Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific
• Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains
• NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines
• Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
• Most instruments will require a 685 LP mirror and 710/20 band pass filter
• NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis
• NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).